DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (C-Term,Arg882) antibody

Antigen: DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A)

Synonyms: DNMT3A, DNMT3A2, M.HsaIIIA, Dnmt3a

Binding Site: C-Term,Arg882

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN658382

Additional Information

Alternative name: DNMT3A

Gene ID: 1788

UniProt: NP_783328.1, Q9Y6K1, NP_072046.2

Immunogen: This DNMT3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human DNMT3A.

Isotype: Ig

Description: Other names: DNA (cytosine-5)-methyltransferase 3A,DNMT3A,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This DNMT3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human DNMT3A.

Molecular Weight: 101858 DA

Comments:

Background: CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq].

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: DNMT3A Antibody (C-term R882) can be refrigerated at 2-8° C for up to 6 months. For long term storage, place the at -20° C.

Research Area: Ubiquitin-related antibodies, Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

DNMT3A Antibody (C-term R882) (ABIN658382) western blot analysis in 293 cell line lysates (35ug/lane). This demonstrates the DNMT3A antibody detected the DNMT3A protein (arrow).

Publications

Product: Holz-Schietinger, Reich: "The inherent processivity of the human de novo methyltransferase 3A (DNMT3A) is enhanced by DNMT3L." in: The Journal of biological chemistry, Vol. 285, Issue 38, pp. 29091-100, 2010 (PubMed).