Lysine (K)-Specific Demethylase 5B (KDM5B) (N-Term) antibody

Antigen: Lysine (K)-Specific Demethylase 5B (KDM5B)

Synonyms: PUT1, Plu1, PLU-1, Rb-Bp2, Jarid1b, AW556288, KIAA4034, RBBP2H1A, mKIAA4034, D1Ertd202e, 2010009J12Rik, 2210016I17Rik, RGD1565602, jarid1b, jarid1ba, id:ibd5050, wu:fd15c05, plu1, cb264, zgc:85741, JARID1B

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN658397

Additional Information

Alternative name: JARID1B

Gene ID: 10765

UniProt: NP_006609.3, Q9UGL1

Immunogen: This JARID1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 191-225 amino acids from the N-terminal region of human JARID1B.

Isotype: Ig

Description: Other names: Lysine-specific demethylase 5B,KDM5B,JARID1B, PLU1, RBBP2H1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This JARID1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 191-225 amino acids from the N-terminal region of human JARID1B.

Molecular Weight: 175658 DA

Comments:

Background: Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma.

Synonyms: PUT1, Plu1, PLU-1, Rb-Bp2, Jarid1b, AW556288, KIAA4034, RBBP2H1A, mKIAA4034, D1Ertd202e, 2010009J12Rik, 2210016I17Rik, RGD1565602, jarid1b, jarid1ba, id:ibd5050, wu:fd15c05, plu1, cb264, zgc:85741, JARID1B

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: JARID1B Antibody (N-term) can be refrigerated at 2-8° C for up to 6 months. For long term storage, place the at -20° C.

Research Area: Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Signaling, Cell Structure

Restrictions: For Research Use only

Images

JARID1B Antibody (N-term) (ABIN658397) western blot analysis in mouse testis tissue lysates (35ug/lane). This demonstrates the JARID1B antibody detected the JARID1B protein (arrow).

Publications

Product: Hayami, Yoshimatsu, Veerakumarasivam et al.: "Overexpression of the JmjC histone demethylase KDM5B in human carcinogenesis: involvement in the proliferation of cancer cells through the E2F/RB pathway." in: Molecular cancer, Vol. 9, pp. 59, 2010 (PubMed).

Yao, Peng, Lin: "The flexible loop L1 of the H3K4 demethylase JARID1B ARID domain has a crucial role in DNA-binding activity." in: Biochemical and biophysical research communications, Vol. 396, Issue 2, pp. 323-8, 2010 (PubMed).

Roesch, Fukunaga-Kalabis, Schmidt et al.: "A temporarily distinct subpopulation of slow-cycling melanoma cells is required for continuous tumor growth." in: Cell, Vol. 141, Issue 4, pp. 583-94, 2010 (PubMed).

Krishnakumar, Kraus: "PARP-1 regulates chromatin structure and transcription through a KDM5B-dependent pathway." in: Molecular cell, Vol. 39, Issue 5, pp. 736-49, 2010 (PubMed).

Kim, Shin, Subramaniam et al.: "Histone demethylase JARID1B/KDM5B is a corepressor of TIEG1/KLF10." in: Biochemical and biophysical research communications, Vol. 401, Issue 3, pp. 412-6, 2010 (PubMed).