|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Lysine (K)-Specific Demethylase 5B (KDM5B) (N-Term) antibody
Alternatives Western Blotting (WB), ELISA
|5 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This JARID1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 191-225 amino acids from the N-terminal region of human JARID1B.|
|Description||Other names: Lysine-specific demethylase 5B,KDM5B,JARID1B, PLU1, RBBP2H1|
|Characteristics||Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This JARID1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 191-225 amino acids from the N-terminal region of human JARID1B.|
|Molecular Weight||175658 DA|
Background: Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma.
|Synonyms||PUT1, Plu1, PLU-1, Rb-Bp2, Jarid1b, AW556288, KIAA4034, RBBP2H1A, mKIAA4034, D1Ertd202e, 2010009J12Rik, 2210016I17Rik, RGD1565602, jarid1b, jarid1ba, id:ibd5050, wu:fd15c05, plu1, cb264, zgc:85741, JARID1B|
|Application Notes||The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||JARID1B Antibody (N-term) can be refrigerated at 2-8° C for up to 6 months. For long term storage, place the at -20° C.|
|Research Area||Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Signaling, Cell Structure|
|Restrictions||For Research Use only|
|JARID1B Antibody (N-term) (ABIN658397) western blot analysis in mouse testis tissue lysates (35ug/lane). This demonstrates the JARID1B antibody detected the JARID1B protein (arrow).|
Hayami, Yoshimatsu, Veerakumarasivam et al.: "Overexpression of the JmjC histone demethylase KDM5B in human carcinogenesis: involvement in the proliferation of cancer cells through the E2F/RB pathway." in: Molecular cancer, Vol. 9, pp. 59, 2010 (PubMed).
Yao, Peng, Lin: "The flexible loop L1 of the H3K4 demethylase JARID1B ARID domain has a crucial role in DNA-binding activity." in: Biochemical and biophysical research communications, Vol. 396, Issue 2, pp. 323-8, 2010 (PubMed).
Roesch, Fukunaga-Kalabis, Schmidt et al.: "A temporarily distinct subpopulation of slow-cycling melanoma cells is required for continuous tumor growth." in: Cell, Vol. 141, Issue 4, pp. 583-94, 2010 (PubMed).
Krishnakumar, Kraus: "PARP-1 regulates chromatin structure and transcription through a KDM5B-dependent pathway." in: Molecular cell, Vol. 39, Issue 5, pp. 736-49, 2010 (PubMed).
Kim, Shin, Subramaniam et al.: "Histone demethylase JARID1B/KDM5B is a corepressor of TIEG1/KLF10." in: Biochemical and biophysical research communications, Vol. 401, Issue 3, pp. 412-6, 2010 (PubMed).
|Hosts||Rabbit (20), Mouse (9)|
|Reactivities||Human (29), Chicken (18), Cow (Bovine) (18), Dog (Canine) (18), Guinea Pig (18), Horse (Equine) (18), Mouse (Murine) (18), Pig (Porcine) (18), Rat (Rattus) (18)|
|Applications||Immunofluorescence (IF) (16), Western Blotting (WB) (12), ELISA (10), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoprecipitation (IP) (2), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||N-Term,AA 191-225 (1)|