Kruppel-Like Factor 1 (erythroid) (KLF1) (C-Term) antibody

Antigen: Kruppel-Like Factor 1 (erythroid) (KLF1)

Synonyms: EKLF, INLU, Eklf, KLF1, MGC142372

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN658420

Additional Information

Alternative name: KLF1

Gene ID: 10661

UniProt: NP_006554.1, Q13351

Immunogen: This KLF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 247-276 amino acids from the C-terminal region of human KLF1.

Isotype: Ig

Description: Other names: Krueppel-like factor 1,KLF1,EKLF

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This KLF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 247-276 amino acids from the C-terminal region of human KLF1.

Molecular Weight: 38221 DA

Comments:

Background: This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: KLF1 Antibody (C-term) can be refrigerated at 2-8° C for up to 6 months. For long term storage, place the at -20° C.

Research Area: Ubiquitin-related antibodies, Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Cell Structure

Restrictions: For Research Use only

Images

KLF1 Antibody (C-term) (ABIN658420) western blot analysis in NCI-H292 cell line lysates (35ug/lane). This demonstrates the KLF1 antibody detected the KLF1 protein (arrow).

Publications

Product: Zhou, Liu, Sun et al.: "KLF1 regulates BCL11A expression and gamma- to beta-globin gene switching." in: Nature genetics, Vol. 42, Issue 9, pp. 742-4, 2010 (PubMed).

Borg, Papadopoulos, Georgitsi et al.: "Haploinsufficiency for the erythroid transcription factor KLF1 causes hereditary persistence of fetal hemoglobin." in: Nature genetics, Vol. 42, Issue 9, pp. 801-5, 2010 (PubMed).