Nonhomologous End-Joining Factor 1 (NHEJ1) (C-Term) antibody

Antigen: Nonhomologous End-Joining Factor 1 (NHEJ1)

Synonyms: XLF, FLJ12610, MGC130191, MGC130192, cernunnos, 1700029B21Rik, NHEJ1, MGC139115, nhej1

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN658424

Additional Information

Alternative name: NHEJ1

Gene ID: 79840

UniProt: NP_079058.1, Q9H9Q4

Immunogen: This NHEJ1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 267-296 amino acids from the C-terminal region of human NHEJ1.

Isotype: Ig

Description: Other names: Non-homologous end-joining factor 1,NHEJ1,XLF

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This NHEJ1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 267-296 amino acids from the C-terminal region of human NHEJ1.

Molecular Weight: 33337 DA

Comments:

Background: Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: NHEJ1 Antibody (C-term) can be refrigerated at 2-8° C for up to 6 months. For long term storage, place the at -20° C.

Research Area: Transcription Factors, Chromatin, Signaling, Cell Structure

Restrictions: For Research Use only

Images

NHEJ1 Antibody (C-term) (ABIN658424) western blot analysis in Hela cell line lysates (35ug/lane). This demonstrates the NHEJ1 antibody detected the NHEJ1 protein (arrow).

Publications

Product: Tsai, Kim, Chu: "Cernunnos/XLF promotes the ligation of mismatched and noncohesive DNA ends." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 19, pp. 7851-6, 2007 (PubMed).

Andres, Modesti, Tsai et al.: "Crystal structure of human XLF: a twist in nonhomologous DNA end-joining." in: Molecular cell, Vol. 28, Issue 6, pp. 1093-101, 2007 (PubMed).

Malivert, Ropars, Nunez et al.: "Delineation of the Xrcc4-interacting region in the globular head domain of cernunnos/XLF." in: The Journal of biological chemistry, Vol. 285, Issue 34, pp. 26475-83, 2010 (PubMed).