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Antibodies
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anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (N-Term) ...
Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (N-Term) antibody
| Antigen |
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| Synonyms |
EIF2AK3 |
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Binding Site
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Conjugate
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Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), PE,Cy5.5 (2), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
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Application
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| Catalog no. |
ABIN658472 |
| Quantity |
0.1 mg (0.25 mg/ml) |
| Price |
280.50 $ Plus shipping costs $45.00
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Bulk discount
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| Shipping to |
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| Availability |
Will be delivered in 2 to 3 Business Days |
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Alternative name
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EIF2AK3
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Gene ID
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9451
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UniProt
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NP_004827.4, Q9NZJ5
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Immunogen
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This EIF2AK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-101 amino acids from the N-terminal region of human EIF2AK3.
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Isotype
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Ig
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Description
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Other names: Eukaryotic translation initiation factor 2-alpha kinase 3,EIF2AK3,PEK, PERK
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Characteristics
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Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
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Specificity
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This EIF2AK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-101 amino acids from the N-terminal region of human EIF2AK3.
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Molecular Weight
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125216 DA
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Comments
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Background: The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome.
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Application Notes
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The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500
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Concentration
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0.25 mg/ml
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Purification
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Purified
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Buffer
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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Storage
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EIF2AK3 Antibody (N-term) can be refrigerated at 2-8° C for up to 6 months. For long term storage, place the at -20° C.
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Research Area
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Translation Factors, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Signaling, Chromatin
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Restrictions
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For Research Use only
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Product
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Kim, Moretti, Mitchell et al.: "Endoplasmic reticulum stress mediates radiation-induced autophagy by perk-eIF2alpha in caspase-3/7-deficient cells." in: Oncogene, Vol. 29, Issue 22, pp. 3241-51, 2010 (PubMed).
Lee, Lee, Lee et al.: "Activation of PERK signaling attenuates Abeta-mediated ER stress." in: PLoS ONE, Vol. 5, Issue 5, pp. e10489, 2010 (PubMed).
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Alternatives for antigen "Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3)", type "Antibodies"
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Hosts
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Rabbit (40), Mouse (4), Goat (1)
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Reactivities
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Human (40), Mouse (Murine) (32), Rat (Rattus) (27), Cow (Bovine) (6), Dog (Canine) (6), Pig (Porcine) (5), Cat (Feline) (1), Chicken (1)
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Applications
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Immunofluorescence (IF) (27), Western Blotting (WB) (23), ELISA (20), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Immunoprecipitation (IP) (6), Immunohistochemistry (IHC) (5), Immunocytochemistry (ICC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), PE,Cy5.5 (2), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
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Epitopes
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pThr980 (5), N-Term (2), AA 29-230 (1), AA 601-1115 (1), C-Term (1), N-Term,Gln163 (1), pSer116 (1), pThr981 (1)
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Alternatives 
2013 BioMed Central Research Awards
