Receptor-Interacting serine-threonine Kinase 3 (RIPK3) (C-Term) antibody

Antigen: Receptor-Interacting serine-threonine Kinase 3 (RIPK3)

Synonyms: RIP3, Hcyp2, RIPK3, MGC152488

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN658475

Additional Information

Alternative name: RIPK3

Gene ID: 11035

UniProt: NP_006862.2, Q9Y572

Immunogen: This RIPK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 479-509 amino acids from the C-terminal region of human RIPK3.

Isotype: Ig

Description: Other names: Receptor-interacting serine/threonine-protein kinase 3,RIPK3,RIP3

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This RIPK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 479-509 amino acids from the C-terminal region of human RIPK3.

Molecular Weight: 56887 DA

Comments:

Background: The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: RIPK3 Antibody (C-term) can be refrigerated at 2-8° C for up to 6 months. For long term storage, place the at -20° C.

Research Area: Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Chromatin, Signaling, Cell Structure

Restrictions: For Research Use only

Images

RIPK3 Antibody (C-term) (ABIN658475) western blot analysis in K562 cell line lysates (35ug/lane). This demonstrates the RIPK3 antibody detected the RIPK3 protein (arrow).

Publications

Product: He, Wang, Miao et al.: "Receptor interacting protein kinase-3 determines cellular necrotic response to TNF-alpha." in: Cell, Vol. 137, Issue 6, pp. 1100-11, 2009 (PubMed).

Goyenechea, Crujeiras, Abete et al.: "Expression of two inflammation-related genes (RIPK3 and RNF216) in mononuclear cells is associated with weight-loss regain in obese subjects." in: Journal of nutrigenetics and nutrigenomics, Vol. 2, Issue 2, pp. 78-84, 2009 (PubMed).