Mediator Complex Subunit 6 (MED6) (AA 135-164), (Center) antibody

Details for Product No. ABIN658616
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Antigen
Epitope
AA 135-164, Center
(8), (8), (8), (8), (5), (3), (2), (1), (1), (1), (1)
Reactivity
Human
(37), (5), (4), (2), (2), (2)
Host
Rabbit
(30), (7)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB)
(34), (22), (4), (2), (2)
Pubmed 5 references available
Quantity 400 μL
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Catalog No. ABIN658616
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Immunogen This MED6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-164 AA from the Central region of human MED6.
Clone RB20183
Isotype Ig
Specificity This MED6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-164 amino acids from the Central region of human MED6.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name MED6
Background Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Synonyms: Mediator of RNA polymerase II transcription subunit 6,MED6,ARC33
Research Area Translation Factors, Signaling, Protein Modifications, Transcription Factors, Cell Structure
Application Notes WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Supplier Images
anti-Mediator Complex Subunit 6 (MED6) (AA 135-164), (Center) antibody MED6 Antibody (Center) (ABIN658616) western blot analysis in WiDr cell line lysates (35 µg/lane). This demonstrates the MED6 antibody detected the MED6 protein (arrow).
Background publications Sato, Tomomori-Sato, Banks et al.: "A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex." in: The Journal of biological chemistry, Vol. 278, Issue 50, pp. 49671-4, 2003 (PubMed).

Tomomori-Sato, Sato, Parmely et al.: "A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2." in: The Journal of biological chemistry, Vol. 279, Issue 7, pp. 5846-51, 2004 (PubMed).

Yang, DeBeaumont, Zhou et al.: "The activator-recruited cofactor/Mediator coactivator subunit ARC92 is a functionally important target of the VP16 transcriptional activator." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 8, pp. 2339-44, 2004 (PubMed).

Sato, Tomomori-Sato, Parmely et al.: "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology." in: Molecular cell, Vol. 14, Issue 5, pp. 685-91, 2004 (PubMed).

Pavri, Lewis, Kim et al.: "PARP-1 determines specificity in a retinoid signaling pathway via direct modulation of mediator." in: Molecular cell, Vol. 18, Issue 1, pp. 83-96, 2005 (PubMed).

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