Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) (Thr45) antibody

Antigen: Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1)

Synonyms: BP-1, 4EBP1, 4E-BP1, PHAS-I, MGC4316, 4e-bp1, AA959816, 153432_at, 43-BP, 4E-BP, 4EBP, BcDNA.HL08053, BcDNA:HL08053, CT8705, Dm 4E-BP, Phas1, anon-WO0172774.158, d4E-BP, d4E-BP1, d4EBP, eIF4E-BP, l(2)k07736, l(2)k13517, pp20, thor, DmelCG8846, CG8846, EIF4EBP1, fc04h09, Eif4ebp1, MGC64137, wu:fc04h09

Binding Site: Thr45

Clonality: Monoclonal

Host: Mouse

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Certificates: ISO 9001:2008

Catalog no.: ABIN658925

Additional Information

Alternative name: 4E-BP1

Gene ID: 1978

UniProt: Q13541

Isotype: IgG

Description: Other names: Phosphorylated heat- and acid-stable protein regulated by insulin 1, PHAS-I, Eukaryotic translation initiation factor 4E-binding protein 1, eIF4E-binding protein 1, 4E-BP1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Molecular Weight: 12580 DA

Comments:

Background: This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Synonyms: BP-1, 4EBP1, 4E-BP1, PHAS-I, MGC4316, 4e-bp1, AA959816, 153432_at, 43-BP, 4E-BP, 4EBP, BcDNA.HL08053, BcDNA:HL08053, CT8705, Dm 4E-BP, Phas1, anon-WO0172774.158, d4E-BP, d4E-BP1, d4EBP, eIF4E-BP, l(2)k07736, l(2)k13517, pp20, thor, DmelCG8846, CG8846, EIF4EBP1, fc04h09, Eif4ebp1, MGC64137, wu:fc04h09

Application Details

Application Notes: The suggested dilution is: WB: 1:500~1:1000 IHC: 1:50-1:100 IF: 1:100-200

Concentration: 1mg/ml

Purification: Purified

Buffer: affinity Purified IgG, in PBS, 0.02% sodium azide and 50% glycerol.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Publications

Product: Villalonga, Fernández de Mattos, Ridley: "RhoE inhibits 4E-BP1 phosphorylation and eIF4E function impairing cap-dependent translation." in: The Journal of biological chemistry, Vol. 284, Issue 51, pp. 35287-96, 2009 (PubMed).

Kumar, Greggio, Beilina et al.: "The Parkinson's disease associated LRRK2 exhibits weaker in vitro phosphorylation of 4E-BP compared to autophosphorylation." in: PLoS ONE, Vol. 5, Issue 1, pp. e8730, 2010 (PubMed).

Aoyagi, Gaspar, Shenk: "Human cytomegalovirus UL69 protein facilitates translation by associating with the mRNA cap-binding complex and excluding 4EBP1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 6, pp. 2640-5, 2010 (PubMed).

Naukkarinen, Surakka, Pietiläinen et al.: "Use of genome-wide expression data to mine the "Gray Zone" of GWA studies leads to novel candidate obesity genes." in: PLoS genetics, Vol. 6, Issue 6, pp. e1000976, 2010 (PubMed).

She, Halilovic, Ye et al.: "4E-BP1 is a key effector of the oncogenic activation of the AKT and ERK signaling pathways that integrates their function in tumors." in: Cancer cell, Vol. 18, Issue 1, pp. 39-51, 2010 (PubMed).