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EIF4EBP1 antibody (Eukaryotic Translation Initiation Factor 4E Binding Protein 1) (Thr45)

Details for Product anti-EIF4EBP1 Antibody No. ABIN658925, Supplier: Login to see
Antigen
  • 153432_at
  • 43-BP
  • 4E-BP
  • 4E-BP1
  • 4EBP
  • 4e-bp
  • 4eBP
  • 4ebp
  • BcDNA.HL08053
  • BcDNA:HL08053
  • CG8846
  • CT8705
  • Dm 4E-BP
  • Dmel\\CG8846
  • Phas1
  • Thor/EIF4EBP
  • anon-WO0172774.158
  • d4E-BP
  • d4E-BP1
  • d4EBP
  • d4e-bp
  • d4eBP
  • dthor
  • eIF4E-BP
  • l(2)06270
  • l(2)k07736
  • l(2)k13517
  • pp20
  • thor
  • EIF4EBP1
  • fc04h09
  • wu:fc04h09
  • zgc:64137
  • 4EBP1
  • BP-1
  • PHAS-I
  • 4e-bp1
  • AA959816
Alternatives
anti-Human EIF4EBP1 antibody for Western Blotting
Epitope
Thr45
59
31
30
22
21
21
17
14
14
14
13
13
13
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11
10
10
8
8
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4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
424
234
185
10
8
5
2
2
1
1
1
Host
Mouse
390
73
9
Clonality
Monoclonal
Conjugate
This EIF4EBP1 antibody is un-conjugated
19
16
11
10
7
7
6
5
5
5
5
5
5
5
5
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
302
197
173
71
57
26
23
18
14
7
6
5
4
3
3
1
Supplier
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Isotype IgG
Specificity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purification Affinity purified
Alternative Name 4E-BP1 (EIF4EBP1 Antibody Abstract)
Background This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Synonyms: Phosphorylated heat- and acid-stable protein regulated by insulin 1, PHAS-I, Eukaryotic translation initiation factor 4E-binding protein 1, eIF4E-binding protein 1, 4E-BP1
Molecular Weight 12580 DA
Gene ID 1978
UniProt Q13541
Research Area Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure
Pathways MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling
Application Notes IHC: 1:50-1:100 , WB: 1:500-1:1000
Comment

Background: This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Restrictions For Research Use only
Format Liquid
Concentration 1mg/ml
Buffer PBS, 0.02% sodium azide and 50% glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications She, Halilovic, Ye et al.: "4E-BP1 is a key effector of the oncogenic activation of the AKT and ERK signaling pathways that integrates their function in tumors." in: Cancer cell, Vol. 18, Issue 1, pp. 39-51, 2010 (PubMed).

Naukkarinen, Surakka, Pietiläinen et al.: "Use of genome-wide expression data to mine the "Gray Zone" of GWA studies leads to novel candidate obesity genes." in: PLoS genetics, Vol. 6, Issue 6, pp. e1000976, 2010 (PubMed).

Aoyagi, Gaspar, Shenk: "Human cytomegalovirus UL69 protein facilitates translation by associating with the mRNA cap-binding complex and excluding 4EBP1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 6, pp. 2640-5, 2010 (PubMed).

Kumar, Greggio, Beilina et al.: "The Parkinson's disease associated LRRK2 exhibits weaker in vitro phosphorylation of 4E-BP compared to autophosphorylation." in: PLoS ONE, Vol. 5, Issue 1, pp. e8730, 2010 (PubMed).

Villalonga, Fernández de Mattos, Ridley: "RhoE inhibits 4E-BP1 phosphorylation and eIF4E function impairing cap-dependent translation." in: The Journal of biological chemistry, Vol. 284, Issue 51, pp. 35287-96, 2009 (PubMed).