Histone H3.1 (HIST1H3B) (Ser10) antibody

Details for Product No. ABIN658963
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Antigen
Synonyms Hist1h2ai, H3 histone, H3.f, H3/f, H3FF, M32460, H3.1-221, H3-H, H3.1, h3f3a, MGC52829, h3.3, h3.3a, H3F3A, H3.3B
Epitope
Ser10
(24), (6), (5), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(76), (55), (51), (2), (1), (1), (1)
Host
Mouse
(69), (10)
Clonality
Monoclonal
Application
Western Blotting (WB), Immunofluorescence (IF)
(75), (34), (33), (18), (16), (4)
Pubmed 5 references available
Catalog no. ABIN658963
Quantity 0.1 mg
Price
464.29 $   Plus shipping costs $45.00
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Isotype IgG
Specificity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purification Affinity purified
Alternative Name Histone H3.1
Background Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Synonyms: Histone H3/l, Histone H3/k, Histone H3/j, Histone H3/i, Histone H3/h, Histone H3/f, Histone H3/d, Histone H3/c, Histone H3/b, Histone H3/a, Histone H3.1
Molecular Weight 15404 DA
Gene ID 8968
UniProt P68431
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure
Application Notes The suggested dilution is: WB: 1:500-1:1000, IF: 1:100-200
Comment

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Restrictions For Research Use only
Format Liquid
Concentration 1mg/ml
Buffer PBS, 0.02% sodium azide and 50% glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Citterio, Papait, Nicassio et al.: "Np95 is a histone-binding protein endowed with ubiquitin ligase activity." in: Molecular and cellular biology, Vol. 24, Issue 6, pp. 2526-35, 2004 (PubMed).

Berndsen, Tsubota, Lindner et al.: "Molecular functions of the histone acetyltransferase chaperone complex Rtt109-Vps75." in: Nature structural & molecular biology, Vol. 15, Issue 9, pp. 948-56, 2009 (PubMed).

Wu, Min, Lunin et al.: "Structural biology of human H3K9 methyltransferases." in: PLoS ONE, Vol. 5, Issue 1, pp. e8570, 2010 (PubMed).

Osakabe, Tachiwana, Matsunaga et al.: "Nucleosome formation activity of human somatic nuclear autoantigenic sperm protein (sNASP)." in: The Journal of biological chemistry, Vol. 285, Issue 16, pp. 11913-21, 2010 (PubMed).

Feng, Yonezawa, Ye et al.: "PHF8 activates transcription of rRNA genes through H3K4me3 binding and H3K9me1/2 demethylation." in: Nature structural & molecular biology, Vol. 17, Issue 4, pp. 445-50, 2010 (PubMed).

Hosts (69), (10)
Reactivities (76), (55), (51), (2), (1), (1), (1)
Applications (75), (34), (33), (18), (16), (4)
Epitopes (24), (6), (5), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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