|+1 404 474 4654|
|+1 888 205 9894 (TF)|
V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) antibody
|Synonyms||BRAF1, RAFB1, B-RAF1, FLJ95109, MGC126806, MGC138284, RMIL, C-RMIL, B-Raf, braf1, rafb1, b-raf1, BRAF|
Alternatives Western Blotting (WB), ELISA
|5 references available|
|Quantity||0.1 mg (0.25 mg/ml) (Variants)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This BRAF Monoclonal antibody is generated from mouse immunized with BRAF recombinant protein.|
|Description||Other names: v-Raf murine sarcoma viral oncogene homolog B1, p94, Proto-oncogene B-Raf, Serine/threonine-protein kinase B-raf, RAFB1, BRAF1, BRAF|
|Characteristics||Purified Mouse Monoclonal Antibody (Mab)|
|Specificity||This BRAF Monoclonal antibody is generated from mouse immunized with BRAF recombinant protein.|
|Molecular Weight||84437 DA|
Background: This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.
|Application Notes||The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:500~16000|
|Buffer||Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Signaling, Protein Modifications|
|Restrictions||For Research Use only|
Hemerly, Bastos, Cerutti: "Identification of several novel non-p.R132 IDH1 variants in thyroid carcinomas." in: European journal of endocrinology / European Federation of Endocrine Societies, Vol. 163, Issue 5, pp. 747-55, 2010 (PubMed).
Moses, Weng, Sansano et al.: "Molecular testing for somatic mutations improves the accuracy of thyroid fine-needle aspiration biopsy." in: World journal of surgery, Vol. 34, Issue 11, pp. 2589-94, 2010 (PubMed).
Irahara, Baba, Nosho et al.: "NRAS mutations are rare in colorectal cancer." in: Diagnostic molecular pathology : the American journal of surgical pathology, part B, Vol. 19, Issue 3, pp. 157-63, 2010 (PubMed).
Wong, Tsang, Deavers et al.: "BRAF mutation is rare in advanced-stage low-grade ovarian serous carcinomas." in: The American journal of pathology, Vol. 177, Issue 4, pp. 1611-7, 2010 (PubMed).
Bruckman, Schönleben, Qiu et al.: "Mutational analyses of the BRAF, KRAS, and PIK3CA genes in oral squamous cell carcinoma." in: Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics, Vol. 110, Issue 5, pp. 632-7, 2010 (PubMed).
|Hosts||Rabbit (34), Mouse (25), Chicken (1)|
|Reactivities||Human (56), Monkey (6), Dog (Canine) (3), Mouse (Murine) (2), Rat (Rattus) (1)|
|Applications||Western Blotting (WB) (46), ELISA (39), Immunohistochemistry (IHC) (25), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), Immunofluorescence (IF) (7), Dot Blot (Dot) (5), Flow Cytometry (FACS) (3), Functional Studies (Func) (1)|
|Epitopes||C-Term (2), Internal Region (2), Ser578 (2), Thr400 (2), pSer445 (2), pSer601 (2), pThr400 (2), pThr439 (2), pThr598 (2), AA 10-22 (1), AA 405-524 (1), AA 754-765 (1), Center, Ser364 (1), N-Term (1), Ser364 (1), Ser428 (1), Ser445 (1), Ser601 (1), Ser728 (1), Thr372 (1), Thr439 (1), Thr598 (1), pThr598, pSer601 (1)|