V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) antibody

Antigen: V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF)

Synonyms: BRAF1, RAFB1, B-RAF1, FLJ95109, MGC126806, MGC138284, RMIL, C-RMIL, B-Raf, braf1, rafb1, b-raf1, BRAF

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN658979

Additional Information

Alternative name: BRAF

Gene ID: 673

UniProt: NP_004324

Immunogen: This BRAF Monoclonal antibody is generated from mouse immunized with BRAF recombinant protein.

Isotype: IgG1

Description: Other names: v-Raf murine sarcoma viral oncogene homolog B1, p94, Proto-oncogene B-Raf, Serine/threonine-protein kinase B-raf, RAFB1, BRAF1, BRAF

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: This BRAF Monoclonal antibody is generated from mouse immunized with BRAF recombinant protein.

Molecular Weight: 84437 DA

Comments:

Background: This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:500~16000

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications

Restrictions: For Research Use only

Publications

Product: Hemerly, Bastos, Cerutti: "Identification of several novel non-p.R132 IDH1 variants in thyroid carcinomas." in: European journal of endocrinology / European Federation of Endocrine Societies, Vol. 163, Issue 5, pp. 747-55, 2010 (PubMed).

Moses, Weng, Sansano et al.: "Molecular testing for somatic mutations improves the accuracy of thyroid fine-needle aspiration biopsy." in: World journal of surgery, Vol. 34, Issue 11, pp. 2589-94, 2010 (PubMed).

Irahara, Baba, Nosho et al.: "NRAS mutations are rare in colorectal cancer." in: Diagnostic molecular pathology : the American journal of surgical pathology, part B, Vol. 19, Issue 3, pp. 157-63, 2010 (PubMed).

Wong, Tsang, Deavers et al.: "BRAF mutation is rare in advanced-stage low-grade ovarian serous carcinomas." in: The American journal of pathology, Vol. 177, Issue 4, pp. 1611-7, 2010 (PubMed).

Bruckman, Schönleben, Qiu et al.: "Mutational analyses of the BRAF, KRAS, and PIK3CA genes in oral squamous cell carcinoma." in: Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics, Vol. 110, Issue 5, pp. 632-7, 2010 (PubMed).