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V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) antibody

Antigen

V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3)
Clonality Monoclonal
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), ELISA
1 reference available
Certificates ISO 9001:2008
Catalog no. ABIN659025
Quantity 0.1 mg  (0.25 mg/ml)
Price 280.50 $   Plus shipping costs $45.00
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Additional Information
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Alternative name AKT3
Gene ID 10000
UniProt NP_859029.1, Q9Y243
Immunogen This AKT3 monoclonal antibody is generated from mouse immunized with AKT3 recombinant protein.
Format Ascites
Isotype IgG1, IgE
Description Other names: STK-2, Protein kinase B gamma, PKB gamma, Protein kinase Akt-3, RAC-PK-gamma, RAC-gamma serine/threonine-protein kinase,PKBG, AKT3
Characteristics Mouse Monoclonal Antibody (Mab)
Specificity This AKT3 monoclonal antibody is generated from mouse immunized with AKT3 recombinant protein.
Molecular Weight 55775  DA
Comments

Background: AKT3 is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1).

Application Details
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Application Notes The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:500~16000
Concentration 0.25 mg/ml
Buffer Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
Storage Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Cell Structure, Protein Modifications, Chromatin, Signaling
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Product Yang, Wang, Chan et al.: "The E3 ligase TRAF6 regulates Akt ubiquitination and activation." in: Science (New York, N.Y.), Vol. 325, Issue 5944, pp. 1134-8, 2009 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3)", type "Antibodies"
Hosts Rabbit (68), Mouse (19), Goat (7), Chicken (1)
Reactivities Human (94), Mouse (Murine) (38), Rat (Rattus) (34), Cow (Bovine) (26), Dog (Canine) (26), Chicken (24), Pig (Porcine) (23), Sheep (Ovine) (23), Cat (Feline) (1)
Applications Western Blotting (WB) (68), ELISA (56), Flow Cytometry (FACS) (23), Immunofluorescence (IF) (23), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), Immunohistochemistry (IHC) (9), Immunoprecipitation (IP) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Fluorescence Microscopy (FM) (2), Dot Blot (Dot) (1), Functional Studies (Func) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), PE,Cy5.5 (2), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes N-Term (6), pSer472 (6), Internal Region (5), AA 1-479 (2), AA 119-131 (2), AA 119-136 (2), C-Term (2), Ser472 (2), Thr447 (2), Transcript Variant 1 (2), pSer166 (1)