|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Alternatives Western Blotting (WB), ELISA
|4 references available|
|Quantity||0.1 mg (0.25 mg/ml) (Variants)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This DPF2 monoclonal antibody is generated from mouse immunized with DPF2 recombinant protein.|
|Isotype||IgG3, kappa chain|
|Description||Other names: Zinc finger protein ubi-d4,DPF2,BAF45D, REQ, UBID4|
|Characteristics||Mouse Monoclonal Antibody (Mab)|
|Specificity||This DPF2 monoclonal antibody is generated from mouse immunized with DPF2 recombinant protein.|
|Molecular Weight||44155 DA|
Background: The protein encoded by this gene is a member of the d4 domain family, characterized by a zinc finger-like structural motif. This protein functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. It likely serves a regulatory role in rapid hematopoietic cell growth and turnover. This gene is considered a candidate gene for multiple endocrine neoplasia type I, an inherited cancer syndrome involving multiple parathyroid, enteropancreatic, and pituitary tumors.
|Application Notes||The suggested dilution is: ELISA~~1:1000 Western blotting~~ 1:125~2000|
|Buffer||Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Apoptosis/Necrosis, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Cell Structure|
|Restrictions||For Research Use only|
Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).
Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).
Matsuyama, Takada, Yokoyama et al.: "Double PHD fingers protein DPF2 recognizes acetylated histones and suppresses the function of estrogen-related receptor alpha through histone deacetylase 1." in: The Journal of biological chemistry, Vol. 285, Issue 24, pp. 18166-76, 2010 (PubMed).
Tando, Ishizaka, Watanabe et al.: "Requiem protein links RelB/p52 and the Brm-type SWI/SNF complex in a noncanonical NF-kappaB pathway." in: The Journal of biological chemistry, Vol. 285, Issue 29, pp. 21951-60, 2010 (PubMed).
|Hosts||Rabbit (41), Mouse (6)|
|Reactivities||Human (47), Mouse (Murine) (27), Rat (Rattus) (26), Chicken (22), Cow (Bovine) (22), Dog (Canine) (22), Xenopus laevis (4), Zebrafish (3), Cat (Feline) (1), Hamster (1)|
|Applications||Western Blotting (WB) (31), ELISA (21), Immunofluorescence (IF) (19), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), Immunohistochemistry (IHC) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Dot Blot (Dot) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||Internal Region (5), N-Term (4), C-Term (3), Center (1)|