DPF2 antibody

Antigen: DPF2

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN659114

Additional Information

Gene ID: 5977

UniProt: NP_006259.1, Q92785

Immunogen: This DPF2 monoclonal antibody is generated from mouse immunized with DPF2 recombinant protein.

Isotype: IgG3, kappa chain

Description: Other names: Zinc finger protein ubi-d4,DPF2,BAF45D, REQ, UBID4

Characteristics: Mouse Monoclonal Antibody (Mab)

Specificity: This DPF2 monoclonal antibody is generated from mouse immunized with DPF2 recombinant protein.

Molecular Weight: 44155 DA

Comments:

Background: The protein encoded by this gene is a member of the d4 domain family, characterized by a zinc finger-like structural motif. This protein functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. It likely serves a regulatory role in rapid hematopoietic cell growth and turnover. This gene is considered a candidate gene for multiple endocrine neoplasia type I, an inherited cancer syndrome involving multiple parathyroid, enteropancreatic, and pituitary tumors.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1000 Western blotting~~ 1:125~2000

Concentration: 0.25 mg/ml

Buffer: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Cell Structure

Restrictions: For Research Use only

Publications

Product: Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Matsuyama, Takada, Yokoyama et al.: "Double PHD fingers protein DPF2 recognizes acetylated histones and suppresses the function of estrogen-related receptor alpha through histone deacetylase 1." in: The Journal of biological chemistry, Vol. 285, Issue 24, pp. 18166-76, 2010 (PubMed).

Tando, Ishizaka, Watanabe et al.: "Requiem protein links RelB/p52 and the Brm-type SWI/SNF complex in a noncanonical NF-kappaB pathway." in: The Journal of biological chemistry, Vol. 285, Issue 29, pp. 21951-60, 2010 (PubMed).