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Details for Product No. ABIN659200

Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) antibody

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Antigen
Synonyms B23, NPM, NO38, Npm, B23NP, CG7917, CRP1, Crp1, Dmel\\CG7917, dNLP, dNLP-S, p22, npm4, npm
Reactivity
»Alternatives Human, Primate
Host
»Alternatives Mouse
Clonality Monoclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Pubmed 2 references available
Catalog no. ABIN659200
Quantity 0.1 mg
Price
330.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Immunogen Purified recombinant fragment of human NPM expressed in E. Coli.
Isotype IgG1
Specificity Purified recombinant fragment of human NPM expressed in E. Coli.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name Nucleophosmin
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 32575 DA
Gene ID 12406
UniProt P06748, NP_954654.1
Research Area Ubiquitin-related antibodies, Translation Factors, Phospho-specific antibodies, Cancer, Signaling, Chromatin, Cell Structure
Application Notes The suggested dilution is: ELISA: ~~ 1:10000. Western Bloting: ~~ 1:500 - 2000. Immunohistochemistry: ~~ 1:200 - 1000. Immunofluorescence: ~~ 1:200 - 1000.
Comment

Background: Nucleophosmin (NPM), also named B23 or NO38, is a major nucleolar protein which is 20 times more abundant in tumor or proliferating cells than in normal resting cells. NPM has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication and molecular chaperoning activities . The NPM is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Morris, Kirstein, Valentine et al.: "Fusion of a kinase gene, ALK, to a nucleolar protein gene, NPM, in non-Hodgkin's lymphoma." in: Science (New York, N.Y.), Vol. 263, Issue 5151, pp. 1281-4, 1994 (PubMed).

Yoneda-Kato, Look, Kirstein et al.: "The t(3;5)(q25.1;q34) of myelodysplastic syndrome and acute myeloid leukemia produces a novel fusion gene, NPM-MLF1." in: Oncogene, Vol. 12, Issue 2, pp. 265-75, 1996 (PubMed).

Alternatives for antigen "Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1)", type "Antibodies"
Hosts (145), (52), (8)
Reactivities (189), (109), (108), (52), (49), (44), (18), (9), (7), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Applications (158), (92), (61), (55), (50), (40), (26), (20), (19), (4), (2), (2), (1), (1)
Conjugates (7), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (1), (1), (1)
Epitopes (30), (25), (17), (12), (12), (11), (6), (6), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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