Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) antibody

Antigen: Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1)

Synonyms: B23, NPM, MGC104254, Npm, NO38, MGC102162, MGC107291, B23NP, MGC108517, npm1, MGC53901, npm

Clonality: Monoclonal

Host: Mouse

Reactivity: Human, Primate

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN659200

Additional Information

Alternative name: Nucleophosmin

Gene ID: 4869

UniProt: P06748, NP_954654.1

Immunogen: Purified recombinant fragment of human NPM expressed in E. Coli.

Isotype: IgG1

Description: Other names: B23, NPM

Characteristics: Mouse Monoclonal antibody to Nucleophosmin

Specificity: Purified recombinant fragment of human NPM expressed in E. Coli.

Molecular Weight: 32575 DA

Comments:

Background: Nucleophosmin (NPM), also named B23 or NO38, is a major nucleolar protein which is 20 times more abundant in tumor or proliferating cells than in normal resting cells. NPM has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication and molecular chaperoning activities . The NPM is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase.

Application Details

Application Notes: The suggested dilution is: ELISA: ~~ 1:10000. Western Bloting: ~~ 1:500 - 2000. Immunohistochemistry: ~~ 1:200 - 1000. Immunofluorescence: ~~ 1:200 - 1000.

Buffer: Purified recombinant fragment of human NPM expressed in E. Coli.

Storage: Nucleophosmin Antibody can be stored at 4?, for long term storage, store at -20?.

Research Area: Ubiquitin-related antibodies, Translation Factors, Phospho-specific antibodies, Cancer, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Morris, Kirstein, Valentine et al.: "Fusion of a kinase gene, ALK, to a nucleolar protein gene, NPM, in non-Hodgkin's lymphoma." in: Science (New York, N.Y.), Vol. 263, Issue 5151, pp. 1281-4, 1994 (PubMed).

Yoneda-Kato, Look, Kirstein et al.: "The t(3;5)(q25.1;q34) of myelodysplastic syndrome and acute myeloid leukemia produces a novel fusion gene, NPM-MLF1." in: Oncogene, Vol. 12, Issue 2, pp. 265-75, 1996 (PubMed).