Apoa5 (ab) antibody

Antigen: Apoa5 (ab)

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunocytochemistry (ICC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN659205

Additional Information

Gene ID: 116519

UniProt: NP_443200.2, Q6Q788

Immunogen: Purified recombinant fragment of human APOA5 expressed in E. Coli.

Isotype: IgG1

Description: Other names: RAP3, APOAV, APOA-V

Characteristics: Mouse Monoclonal antibody to Apoa5(ab)

Specificity: Purified recombinant fragment of human APOA5 expressed in E. Coli.

Molecular Weight: 41213 DA

Comments:

Background: Apolipoprotein A5 (ApoA5) is fast gaining attention as a key regulator of serum triglyceride concentrations. An ApoA5 mouse knock-out model produced an approximately four fold increase in serum triglycerides, whereas a knock-in model with human ApoA5 produced 50ﾖ70% lower concentrations of mouse serum triglycerides. In addition, peroxisome proliferator-activated receptor-_ agonists, which are used clinically to lower serum triglyceride concentrations, cause increased ApoA5 mRNA expression. Recently, it was demonstrated that ApoA5 is present in human serum detected by polyclonal antibodies against both the NH2 and COOH termini, although at much lower concentration than other apolipoproteins.

Application Details

Application Notes: The suggested dilution is: ELISA: ~~ 1:10000. Western Bloting: ~~ 1:500 - 2000.

Buffer: Purified recombinant fragment of human APOA5 expressed in E. Coli.

Storage: Apoa5(ab) Antibody can be stored at 4?, for long term storage, store at -20?.

Research Area: Phospho-specific antibodies, Cell Structure

Restrictions: For Research Use only

Publications

Product: Pennacchio, Olivier, Hubacek et al.: "An apolipoprotein influencing triglycerides in humans and mice revealed by comparative sequencing." in: Science (New York, N.Y.), Vol. 294, Issue 5540, pp. 169-73, 2001 (PubMed).