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CHEK1 antibody (Checkpoint Kinase 1)

Details for Product anti-CHEK1 Antibody No. ABIN659229, Supplier: Login to see New
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(454), (140), (130), (4), (4), (3), (3), (2), (2), (2), (1), (1)
(397), (49), (23), (6)
This CHEK1 antibody is un-conjugated
(12), (12), (11), (8), (8), (8), (5), (3), (3), (3), (3), (3), (3), (3), (3)
Western Blotting (WB)
(374), (174), (125), (59), (57), (36), (26), (22), (22), (8), (5), (4), (2), (2)
Pubmed 1 reference available
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Catalog number from supplier Login to see New
Quantity 0.1 mg
Shipping to United States ( )
Immunogen Purified recombinant fragment of CHK1 expressed in E. Coli.
Isotype IgG1
Specificity Purified recombinant fragment of CHK1 expressed in E. Coli.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name CHK1 (CHEK1 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 54420 DA
Gene ID 12406
NCBI Accession NP_001265
UniProt O14757
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Signaling, Chromatin, Cell Structure
Pathways p53 Signaling, Apoptosis, Cell Division Cycle
Application Notes Western Blot: = 1:500 - 2, 000

Background: CHK1, also known as CHEK1, it is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Cdc25, when phosphorylated on serine 216 by chk1 becomes bound by an adaptor protein in the cytoplasm. Therefore it is inhibited from removing the inhibiting phosphate from MPF (mitotic/maturation promoting factor) added by Wee1. Consequently, a cell is prevented from entering mitosis. Chk1 can also phosphorylate p53 at Ser20 in vitro.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Matsuoka, Huang, Elledge: "Linkage of ATM to cell cycle regulation by the Chk2 protein kinase." in: Science (New York, N.Y.), Vol. 282, Issue 5395, pp. 1893-7, 1998 (PubMed).

Catalog No. ABIN659229

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