Add to Basket Bulk discount
Order hotline:
+1 404 474 4654
+1 888 205 9894 (TF)

Checkpoint Kinase 1 (CHEK1) antibody

Antigen

Checkpoint Kinase 1 (CHEK1)
Synonyms CHK1, Chk1, rad27, C85740, CHEK1, LOC398191, chk1, chek1
Clonality Monoclonal
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), ELISA
1 reference available
Certificates ISO 9001:2008
Catalog no. ABIN659229
Quantity 0.1mg
Price 330.00 $   Plus shipping costs $45.00
Bulk discount
Shipping to
Availability Will be delivered in 2 to 3 Business Days

Additional Information
Application Details Publications Alternatives back to top

Alternative name CHK1
Gene ID 1111
UniProt NP_001265.2, O14757
Immunogen Purified recombinant fragment of CHK1 expressed in E. Coli.
Isotype IgG1
Description Other names: CHEK1
Characteristics Mouse Monoclonal Antibody to CHK1
Specificity Purified recombinant fragment of CHK1 expressed in E. Coli.
Molecular Weight 54420 DA
Comments

Background: CHK1, also known as CHEK1, it is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Cdc25, when phosphorylated on serine 216 by chk1 becomes bound by an adaptor protein in the cytoplasm. Therefore it is inhibited from removing the inhibiting phosphate from MPF (mitotic/maturation promoting factor) added by Wee1. Consequently, a cell is prevented from entering mitosis. Chk1 can also phosphorylate p53 at Ser20 in vitro.

Application Details
Publications Alternatives back to top

Application Notes The suggested dilution is: ELISA: ~~ 1:10,000. Western Blot: ~~ 1:500 - 2,000
Buffer Purified recombinant fragment of CHK1 expressed in E. Coli.
Storage CHK1 Antibody can be stored at 4?, for long term storage, store at -20?.
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Signaling, Chromatin, Cell Structure
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Product Matsuoka, Huang, Elledge: "Linkage of ATM to cell cycle regulation by the Chk2 protein kinase." in: Science (New York, N.Y.), Vol. 282, Issue 5395, pp. 1893-7, 1998 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Checkpoint Kinase 1 (CHEK1)", type "Antibodies"
Hosts Rabbit (174), Mouse (19), Goat (6), Sheep (4)
Reactivities Human (192), Rat (Rattus) (82), Mouse (Murine) (71), Horse (Equine) (10), Cow (Bovine) (9), Chicken (8), Dog (Canine) (7), Pig (Porcine) (6), Monkey (2), Xenopus laevis (2), Yeast (Saccharomyces cerevisiae) (2), Zebrafish (2), Cat (Feline) (1), Rabbit (1), Yeast (1), Zebrafish (Danio rerio) (1)
Applications Western Blotting (WB) (129), ELISA (80), Immunofluorescence (IF) (60), Immunohistochemistry (IHC) (43), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (32), Immunoprecipitation (IP) (15), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunocytochemistry (ICC) (5), Dot Blot (Dot) (2), Flow Cytometry (FACS) (2), Immunoelectron Microscopy (IEM) (2)
Conjugates Alexa Fluor 350 (5), Alexa Fluor 488 (5), Alexa Fluor 555 (5), Alexa Fluor 647 (5), PE,Cy5.5 (4), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)
Epitopes pSer317 (23), pSer280 (20), Ser296 (17), pSer345 (16), Internal Region (7), pSer296 (7), C-Term (6), Ser280 (4), AA 312-327 (3), N-Term (3), Center (2), AA 1-474 (1), AA 250-300 (1), AA 309-321 (1), Middle Region (1), pSer286 (1), pSer301 (1), pThr14 (1), pThr29 (1)