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Details for Product No. ABIN659241

RAB25, Member RAS Oncogene Family (RAB25) antibody

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Antigen
Synonyms CATX-8, RAB11C, AV012978, MGC84182, RAB25, catx-8, MGC79587, rab25, si:dkeyp-84f11.7, zgc:101648
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality Monoclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Pubmed 2 references available
Catalog no. ABIN659241
Quantity 0.1 mg
Price
423.50 $   Plus shipping costs $45.00
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Immunogen Purified recombinant fragment of Rab25 expressed in E. Coli.
Isotype IgG2a
Specificity Purified recombinant fragment of Rab25 expressed in E. Coli.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name Rab25
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 23496 DA
Gene ID 12406
UniProt NP_065120.2, P57735
Research Area Signaling, Chromatin, Cell Structure
Application Notes The suggested dilution is: ELISA: ~~ 1:10000. Western Bloting: ~~ 1:500 - 2000. Immunohistochemistry: ~~ 1:200 - 1000.
Comment

Background: Members of the Ras-related superfamily of GTP binding proteins, which includes Ras, Rho, Rab and ARF subfamilies, exhibit 30-50% similarity with Ras p21. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% similar to Rab proteins, is associated with secretory vesicles. Rab proteins located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Rab25 was cloned from a gastric parietal cell cDNA library and is expressed in epithelial tissues such as the gastrointestinal mucosae, kidney, and lung, which encoded a protein of 28 kDa

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Goldenring, Shen, Vaughan et al.: "Identification of a small GTP-binding protein, Rab25, expressed in the gastrointestinal mucosa, kidney, and lung." in: The Journal of biological chemistry, Vol. 268, Issue 25, pp. 18419-22, 1993 (PubMed).

Wang, Kumar, Navarre et al.: "Regulation of vesicle trafficking in madin-darby canine kidney cells by Rab11a and Rab25." in: The Journal of biological chemistry, Vol. 275, Issue 37, pp. 29138-46, 2000 (PubMed).

Alternatives for antigen "RAB25, Member RAS Oncogene Family (RAB25)", type "Antibodies"
Hosts (21), (16), (3)
Reactivities (39), (23), (15), (14), (14), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Applications (31), (18), (11), (10), (8), (3), (3), (3), (3), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (2), (2), (2), (2), (2), (2), (1)
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