RAB25, Member RAS Oncogene Family (RAB25) antibody

Antigen: RAB25, Member RAS Oncogene Family (RAB25)

Synonyms: CATX-8, RAB11C, AV012978, MGC84182, RAB25, catx-8, MGC79587, zgc:101648, si:dkeyp-84f11.7, MGC140813

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN659241

Additional Information

Alternative name: Rab25

Gene ID: 57111

UniProt: NP_065120.2, P57735

Immunogen: Purified recombinant fragment of Rab25 expressed in E. Coli.

Isotype: IgG2a

Description: Other names: CATX-8, RAB25

Characteristics: Mouse Monoclonal Antibody to Rab25

Specificity: Purified recombinant fragment of Rab25 expressed in E. Coli.

Molecular Weight: 23496 DA

Comments:

Background: Members of the Ras-related superfamily of GTP binding proteins, which includes Ras, Rho, Rab and ARF subfamilies, exhibit 30-50% similarity with Ras p21. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% similar to Rab proteins, is associated with secretory vesicles. Rab proteins located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Rab25 was cloned from a gastric parietal cell cDNA library and is expressed in epithelial tissues such as the gastrointestinal mucosae, kidney, and lung, which encoded a protein of 28 kDa

Application Details

Application Notes: The suggested dilution is: ELISA: ~~ 1:10000. Western Bloting: ~~ 1:500 - 2000. Immunohistochemistry: ~~ 1:200 - 1000.

Buffer: Purified recombinant fragment of Rab25 expressed in E. Coli.

Storage: Rab25 Antibody can be stored at 4?, for long term storage, store at -20?.

Research Area: Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Goldenring, Shen, Vaughan et al.: "Identification of a small GTP-binding protein, Rab25, expressed in the gastrointestinal mucosa, kidney, and lung." in: The Journal of biological chemistry, Vol. 268, Issue 25, pp. 18419-22, 1993 (PubMed).

Wang, Kumar, Navarre et al.: "Regulation of vesicle trafficking in madin-darby canine kidney cells by Rab11a and Rab25." in: The Journal of biological chemistry, Vol. 275, Issue 37, pp. 29138-46, 2000 (PubMed).