Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) antibody

Antigen: Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1)

Synonyms: BP-1, 4EBP1, 4E-BP1, PHAS-I, MGC4316, 4e-bp1, AA959816, 153432_at, 43-BP, 4E-BP, 4EBP, BcDNA.HL08053, BcDNA:HL08053, CT8705, Dm 4E-BP, Phas1, anon-WO0172774.158, d4E-BP, d4E-BP1, d4EBP, eIF4E-BP, l(2)k07736, l(2)k13517, pp20, thor, DmelCG8846, CG8846, EIF4EBP1, fc04h09, Eif4ebp1, MGC64137, wu:fc04h09

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN659287

Additional Information

Alternative name: 4E-BP1

Gene ID: 1978

UniProt: Q13541, NP_004086.1

Immunogen: Purified recombinant fragment of 4EBP1 expressed in E. Coli.

Isotype: IgG1

Description: Other names: BP-1, 4EBP1, 4E-BP1, PHAS-I, MGC4316, EIF4EBP1

Characteristics: Mouse Monoclonal Antibody to 4E-BP1

Specificity: Purified recombinant fragment of 4EBP1 expressed in E. Coli.

Molecular Weight: 12580 DA

Comments:

Background: 4E-BP1(eukaryotic translation Initiation Factor 4E Binding Protein 1),also called ELF4EBP1/BP-1/PHAS-I ,which is located on chromosome 8p12, with 118-amino acid protein (about 13kDa). Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while(24kDa), the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction.

Synonyms: BP-1, 4EBP1, 4E-BP1, PHAS-I, MGC4316, 4e-bp1, AA959816, 153432_at, 43-BP, 4E-BP, 4EBP, BcDNA.HL08053, BcDNA:HL08053, CT8705, Dm 4E-BP, Phas1, anon-WO0172774.158, d4E-BP, d4E-BP1, d4EBP, eIF4E-BP, l(2)k07736, l(2)k13517, pp20, thor, DmelCG8846, CG8846, EIF4EBP1, fc04h09, Eif4ebp1, MGC64137, wu:fc04h09

Application Details

Application Notes: The suggested dilution is: ELISA: ~~ 1:10000. Western Bloting: ~~ 1:500 - 2000.

Buffer: Purified recombinant fragment of 4EBP1 expressed in E. Coli.

Storage: 4E-BP1 Antibody can be stored at 4?, for long term storage, store at -20?.

Research Area: Translation Factors, Phospho-specific antibodies, Signaling, Cell Structure

Restrictions: For Research Use only

Publications

Product: Pause, Belsham, Gingras et al.: "Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5'-cap function." in: Nature, Vol. 371, Issue 6500, pp. 762-7, 1994 (PubMed).

Fadden, Haystead, Lawrence: "Identification of phosphorylation sites in the translational regulator, PHAS-I, that are controlled by insulin and rapamycin in rat adipocytes." in: The Journal of biological chemistry, Vol. 272, Issue 15, pp. 10240-7, 1997 (PubMed).