anti-EIF4EBP1 antibody (Eukaryotic Translation Initiation Factor 4E Binding Protein 1)

Details for Product anti-EIF4EBP1 Antibody No. ABIN659289
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(451), (268), (200), (5), (3), (3), (2), (1), (1), (1)
(418), (75), (27)
This EIF4EBP1 antibody is un-conjugated
(27), (23), (16), (12), (8), (8), (6), (6), (6), (6), (6), (6), (6), (6), (2), (2)
Immunohistochemistry (IHC), Western Blotting (WB)
(328), (221), (205), (77), (60), (40), (28), (17), (16), (9), (6), (6), (5), (5), (4), (3), (1)
Pubmed 2 references available
Quantity 0.1 mg
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Immunogen Purified recombinant fragment of 4EBP1 expressed in E. Coli.
Isotype IgG1
Specificity Purified recombinant fragment of 4EBP1 expressed in E. Coli.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name 4E-BP1 (EIF4EBP1 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 12580 DA
Gene ID 12406
NCBI Accession NP_004086
UniProt Q13541
Research Area Translation Factors, Phospho-specific antibodies, Signaling, Cell Structure
Pathways MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling
Application Notes Western Bloting: = 1:500 - 2000, Immunohistochemistry: = 1:200 - 1000.

Background: 4E-BP1(eukaryotic translation Initiation Factor 4E Binding Protein 1),also called ELF4EBP1/BP-1/PHAS-I ,which is located on chromosome 8p12, with 118-amino acid protein (about 13kDa). Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while(24kDa), the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Fadden, Haystead, Lawrence: "Identification of phosphorylation sites in the translational regulator, PHAS-I, that are controlled by insulin and rapamycin in rat adipocytes." in: The Journal of biological chemistry, Vol. 272, Issue 15, pp. 10240-7, 1997 (PubMed).

Pause, Belsham, Gingras et al.: "Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5'-cap function." in: Nature, Vol. 371, Issue 6500, pp. 762-7, 1994 (PubMed).

Catalog No. ABIN659289
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