V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) antibody

Details for Product No. ABIN659292
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(347), (43), (32), (1), (1), (1), (1)
(256), (83), (5), (3)
(21), (21), (21), (18), (18), (18), (3), (3), (3), (3), (3), (3), (3), (3)
Immunohistochemistry (IHC), Western Blotting (WB)
(240), (176), (142), (82), (51), (40), (30), (27), (12), (11), (4), (2), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Immunogen Purified recombinant fragment of BRAF expressed in E. Coli.
Isotype IgG1
Specificity Purified recombinant fragment of BRAF expressed in E. Coli.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name BRAF (BRAF Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 84437 DA
Gene ID 12406
NCBI Accession NP_004324
UniProt P15056
Research Area Phospho-specific antibodies, Cell Signaling, Cancer, Signaling, Chromatin, Cell Structure
Pathways MAPK Pathway, RTK signaling
Application Notes Western Bloting: = 1:500 - 2000, Immunohistochemistry: = 1:200 - 1000.

Background: BRAF(V-raf murine sarcoma viral oncogene homolog B1 ) is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439).B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK),it has a long amino-terminal region,the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers.The BRAF gene mutation is frequently detected in papillary thyroid carcinoma,melanocytic nevi,primary cutaneous melanomas and colorectal cancers.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Kim, Giuliano, Turner et al.: "Lymphatic mapping establishes the role of BRAF gene mutation in papillary thyroid carcinoma." in: Annals of surgery, Vol. 244, Issue 5, pp. 799-804, 2006 (PubMed).

Noda, Kato, Yoshikawa et al.: "Frequent involvement of ras-signalling pathways in both polypoid-type and flat-type early-stage colorectal cancers." in: Journal of experimental & clinical cancer research : CR, Vol. 25, Issue 2, pp. 235-42, 2006 (PubMed).

Terai, Matsuda: "The amino-terminal B-Raf-specific region mediates calcium-dependent homo- and hetero-dimerization of Raf." in: The EMBO journal, Vol. 25, Issue 15, pp. 3556-64, 2006 (PubMed).

Fullen, Poynter, Lowe et al.: "BRAF and NRAS mutations in spitzoid melanocytic lesions." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 19, Issue 10, pp. 1324-32, 2006 (PubMed).

Rapp, Goldsborough, Mark et al.: "Structure and biological activity of v-raf, a unique oncogene transduced by a retrovirus." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, Issue 14, pp. 4218-22, 1983 (PubMed).

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Catalog No. ABIN659292
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