Add to Basket Bulk discount
Order hotline:
+1 404 474 4654
+1 888 205 9894 (TF)

Bruton Agammaglobulinemia tyrosine Kinase (BTK) antibody

Antigen

Bruton Agammaglobulinemia tyrosine Kinase (BTK)
Synonyms BTK, atk, bpk, xla, imd1, agmx1, psctk1
Clonality Monoclonal
Host
Alternatives

Mouse
Reactivity
Alternatives

Human, Primate
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), ELISA
2 references available
Certificates ISO 9001:2008
Catalog no. ABIN659312
Quantity 0.1ml
Price 357.50 $   Plus shipping costs $45.00
Bulk discount
Shipping to
Availability Will be delivered in 2 to 3 Business Days

Additional Information
Application Details Publications Alternatives back to top

Alternative name BTK
Gene ID 695
UniProt NP_000052.1, Q06187
Immunogen Purified recombinant fragment of BTK expressed in E. Coli.
Isotype IgG1
Description Other names: BTK
Characteristics Mouse Monoclonal Antibody to BTK
Specificity Purified recombinant fragment of BTK expressed in E. Coli.
Molecular Weight 76281 DA
Comments

Background: Brutons tyrosine kinase (BTK) is a member of the BTK/Tec family of cytoplasmic tyrosine kinases.All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini.BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation.

Application Details
Publications Alternatives back to top

Application Notes The suggested dilution is: ELISA: ~~ 1:10000. Western Bloting: ~~ 1:500 - 2000. Immunohistochemistry: ~~ 1:200 - 1000. Immunofluorescence: ~~ 1:200 - 1000.
Buffer Purified recombinant fragment of BTK expressed in E. Coli.
Storage BTK Antibody can be stored at 4?, for long term storage, store at -20?.
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Cell Structure, Chromatin, Signaling
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Product Tamagnone, Lahtinen, Mustonen et al.: "BMX, a novel nonreceptor tyrosine kinase gene of the BTK/ITK/TEC/TXK family located in chromosome Xp22.2." in: Oncogene, Vol. 9, Issue 12, pp. 3683-8, 1994 (PubMed).

Yamada, Kawakami, Kimura et al.: "Structure and expression of novel protein-tyrosine kinases, Emb and Emt, in hematopoietic cells." in: Biochemical and biophysical research communications, Vol. 192, Issue 1, pp. 231-40, 1993 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Bruton Agammaglobulinemia tyrosine Kinase (BTK)", type "Antibodies"
Hosts Rabbit (52), Mouse (15)
Reactivities Human (68), Mouse (Murine) (38), Rat (Rattus) (36), Cow (Bovine) (29), Horse (Equine) (28), Pig (Porcine) (28), Dog (Canine) (24), Monkey (2), Cat (Feline) (1), Chicken (1)
Applications Western Blotting (WB) (42), Immunofluorescence (IF) (39), ELISA (23), Immunohistochemistry (IHC) (15), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Flow Cytometry (FACS) (9), Immunocytochemistry (ICC) (6), Immunoprecipitation (IP) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), PE,Cy5.5 (3), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pTyr223 (6), pSer180 (5), N-Term (4), Internal Region (2), pTyr551 (2), C-Term (1), Center (1), pThr514 (1), pTyr96 (1)