Bruton Agammaglobulinemia tyrosine Kinase (BTK) antibody

Details for Product No. ABIN659312
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Antigen
Synonyms BTK, atk, bpk, xla, imd1, agmx1, psctk1, AGMX1, AT, ATK, BPK, IMD1, PSCTK1, XLA, AI528679, xid
Reactivity
Human, Primate
(192), (79), (70), (36), (36), (24), (8)
Host
Mouse
(135), (56)
Clonality
Monoclonal
Conjugate
Un-conjugated
(7), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
(154), (66), (64), (55), (46), (40), (34), (29), (4), (4), (3), (2)
Pubmed 2 references available
Quantity 0.1 mg
Options
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Catalog No. ABIN659312
357.50 $
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Immunogen Purified recombinant fragment of BTK expressed in E. Coli.
Isotype IgG1
Specificity Purified recombinant fragment of BTK expressed in E. Coli.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name BTK
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 76281 DA
Gene ID 12406
NCBI Accession NP_000052
UniProt Q06187
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Cell Structure, Chromatin, Signaling
Application Notes The suggested dilution is: Western Bloting: = 1:500 - 2000. Immunohistochemistry: = 1:200 - 1000. Immunofluorescence: = 1:200 - 1000.
Comment

Background: Brutons tyrosine kinase (BTK) is a member of the BTK/Tec family of cytoplasmic tyrosine kinases.All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini.BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Tamagnone, Lahtinen, Mustonen et al.: "BMX, a novel nonreceptor tyrosine kinase gene of the BTK/ITK/TEC/TXK family located in chromosome Xp22.2." in: Oncogene, Vol. 9, Issue 12, pp. 3683-8, 1994 (PubMed).

Yamada, Kawakami, Kimura et al.: "Structure and expression of novel protein-tyrosine kinases, Emb and Emt, in hematopoietic cells." in: Biochemical and biophysical research communications, Vol. 192, Issue 1, pp. 231-40, 1993 (PubMed).

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