ER-A antibody

Details for Product No. ABIN659319
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Antigen
Reactivity
Human
(2)
Host
Mouse
(1)
Clonality
Monoclonal
Application
Western Blotting (WB)
(2), (2)
Pubmed 2 references available
Quantity 0.1 mg
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Immunogen Purified recombinant fragment of ER expressed in E. Coli.
Isotype IgG2b
Specificity Purified recombinant fragment of ER expressed in E. Coli.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ER
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 66216 DA
Gene ID 12406
NCBI Accession NP_001116214
UniProt P03372
Research Area Ubiquitin-related antibodies, Translation Factors, Phospho-specific antibodies, Transcription Factors, Signaling, Cell Structure
Application Notes Western Bloting: = 1:500 - 2000.
Comment

Background: ER (estrogen receptor 1) a member of the steroid receptor superfamily, contains highly conserved DNA binding (DBD) and ligand binding domains (LBD). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ER regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery. Phosphorylation provides an important mechanism to regulate ER activity. ER is phosphorylated on multiple sites. Serines 104, 106, 118 and 167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serines plays an important role in regulating ER activity. Ser118 may be the substrate of the transcription regulatory kinase cdK7. Ser167 may be phosphorylated by p90RSK and Akt. Phosphorylation of Ser167 may confer tamoxifen resistance in breast cancer patients.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Campbell, Bhat-Nakshatri, Patel et al.: "Phosphatidylinositol 3-kinase/AKT-mediated activation of estrogen receptor alpha: a new model for anti-estrogen resistance." in: The Journal of biological chemistry, Vol. 276, Issue 13, pp. 9817-24, 2001 (PubMed).

Chen, Riedl, Washbrook et al.: "Activation of estrogen receptor alpha by S118 phosphorylation involves a ligand-dependent interaction with TFIIH and participation of CDK7." in: Molecular cell, Vol. 6, Issue 1, pp. 127-37, 2000 (PubMed).

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Catalog No. ABIN659319
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