Coagulation Factor II (Thrombin) Receptor-Like 3 (F2RL3) antibody

Antigen: Coagulation Factor II (Thrombin) Receptor-Like 3 (F2RL3)

Synonyms: F2RL3, MGC140530, F2, DKFZp470K2111, DKFZp470L0512

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN659384

Additional Information

Alternative name: PAR4

Gene ID: 5074

UniProt: Q96IZ0, NP_002574.2

Immunogen: Purified recombinant fragment of PAR4(aa1-330) expressed in E. Coli.

Isotype: IgG1

Description: Other names: PAWR

Characteristics: Mouse Monoclonal Antibody to PAR4

Specificity: Purified recombinant fragment of PAR4(aa1-330) expressed in E. Coli.

Molecular Weight: 36568 DA

Comments:

Background: Prostate apoptosis response 4 (Par4) is a 38kD protein originally identified as the product of a gene that is upregulated in prostate tumor cells undergoing apoptosis. It is a leucine zipper and death domain containing protein whose levels increase in neurons undergoing apoptosis as a result of trophic factor withdrawal or exposure to oxidative and metabolic insults. Par4 levels are reported to be increased in their lumbar spinal cord specimens further suggesting a role in neuronal degeneration. The tumor suppressor WT1 represses and activates transcription. The loss and/or imbalance of the dual transcriptional activity of WT1 may contribute to Wilms tumor. Par4 is a WT1 interacting protein that also functions as a transcriptional repressor.

Application Details

Application Notes: The suggested dilution is: ELISA: ~~ 1:10000. Western Bloting: ~~ 1:500 - 2000. Immunohistochemistry: ~~ 1:200 - 1000.

Buffer: Purified recombinant fragment of PAR4(aa1-330) expressed in E. Coli.

Storage: PAR4 Antibody can be stored at 4?, for long term storage, store at -20?.

Research Area: Apoptosis/Necrosis, Phospho-specific antibodies, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Publications

Product: Xie, Guo: "Par-4 inhibits choline uptake by interacting with CHT1 and reducing its incorporation on the plasma membrane." in: The Journal of biological chemistry, Vol. 279, Issue 27, pp. 28266-75, 2004 (PubMed).

Kukoc-Zivojnov, Puccetti, Chow et al.: "Prostate apoptosis response gene-4 (par-4) abrogates the survival function of p185(BCR-ABL) in hematopoietic cells." in: Experimental hematology, Vol. 32, Issue 7, pp. 649-56, 2004 (PubMed).

Gurumurthy, Goswami, Vasudevan et al.: "Phosphorylation of Par-4 by protein kinase A is critical for apoptosis." in: Molecular and cellular biology, Vol. 25, Issue 3, pp. 1146-61, 2005 (PubMed).

Weller, Dahl, Lohoff et al.: "No association between polymorphisms in the prostate apoptosis factor-4 gene and cocaine dependence." in: Psychiatric genetics, Vol. 16, Issue 5, pp. 193-6, 2006 (PubMed).