|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Coagulation Factor II (Thrombin) Receptor-Like 3 (F2RL3) antibody
|Synonyms||F2RL3, MGC140530, F2, DKFZp470K2111, DKFZp470L0512|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|4 references available|
|Price||423.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||Purified recombinant fragment of PAR4(aa1-330) expressed in E. Coli.|
|Description||Other names: PAWR|
|Characteristics||Mouse Monoclonal Antibody to PAR4|
|Specificity||Purified recombinant fragment of PAR4(aa1-330) expressed in E. Coli.|
|Molecular Weight||36568 DA|
Background: Prostate apoptosis response 4 (Par4) is a 38kD protein originally identified as the product of a gene that is upregulated in prostate tumor cells undergoing apoptosis. It is a leucine zipper and death domain containing protein whose levels increase in neurons undergoing apoptosis as a result of trophic factor withdrawal or exposure to oxidative and metabolic insults. Par4 levels are reported to be increased in their lumbar spinal cord specimens further suggesting a role in neuronal degeneration. The tumor suppressor WT1 represses and activates transcription. The loss and/or imbalance of the dual transcriptional activity of WT1 may contribute to Wilms tumor. Par4 is a WT1 interacting protein that also functions as a transcriptional repressor.
|Application Notes||The suggested dilution is: ELISA: ~~ 1:10000. Western Bloting: ~~ 1:500 - 2000. Immunohistochemistry: ~~ 1:200 - 1000.|
|Buffer||Purified recombinant fragment of PAR4(aa1-330) expressed in E. Coli.|
|Storage||PAR4 Antibody can be stored at 4?, for long term storage, store at -20?.|
|Research Area||Apoptosis/Necrosis, Phospho-specific antibodies, Transcription Factors, Signaling, Cell Structure|
|Restrictions||For Research Use only|
Xie, Guo: "Par-4 inhibits choline uptake by interacting with CHT1 and reducing its incorporation on the plasma membrane." in: The Journal of biological chemistry, Vol. 279, Issue 27, pp. 28266-75, 2004 (PubMed).
Kukoc-Zivojnov, Puccetti, Chow et al.: "Prostate apoptosis response gene-4 (par-4) abrogates the survival function of p185(BCR-ABL) in hematopoietic cells." in: Experimental hematology, Vol. 32, Issue 7, pp. 649-56, 2004 (PubMed).
Gurumurthy, Goswami, Vasudevan et al.: "Phosphorylation of Par-4 by protein kinase A is critical for apoptosis." in: Molecular and cellular biology, Vol. 25, Issue 3, pp. 1146-61, 2005 (PubMed).
Weller, Dahl, Lohoff et al.: "No association between polymorphisms in the prostate apoptosis factor-4 gene and cocaine dependence." in: Psychiatric genetics, Vol. 16, Issue 5, pp. 193-6, 2006 (PubMed).
|Hosts||Rabbit (79), Mouse (3), Chicken (1)|
|Reactivities||Human (82), Mouse (Murine) (65), Rat (Rattus) (46), Dog (Canine) (19), Horse (Equine) (19), Cow (Bovine) (18), Guinea Pig (18), Pig (Porcine) (18)|
|Applications||Immunofluorescence (IF) (49), Western Blotting (WB) (29), ELISA (21), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (17), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (9), Immunoprecipitation (IP) (7), Immunohistochemistry (IHC) (5), Immunoelectron Microscopy (IEM) (3), Flow Cytometry (FACS) (1)|
|Conjugates||Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), Biotin (3), Cy3 (3), Cy5 (3), Cy5.5 (3), Cy7 (3), FITC (3), Gold (3), HRP (3), PE (3), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1)|
|Epitopes||C-Term (21), pThr163 (18), Internal Region (4), N-Term (3), AA 777-986 (1), Cleaved-Gly48 (1), Cytoplasmic Domain (1), Extracellular Domain (1)|