Lysyl-tRNA Synthetase (KARS) (AA 90-174) antibody

Details for Product No. ABIN659402
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Antigen
Epitope
AA 90-174
(7), (5), (4), (4), (1), (1)
Reactivity
Human
(45), (20), (17), (1)
Host
Mouse
(36), (10)
Clonality
Monoclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(35), (17), (17), (11), (10), (6), (5), (4), (3), (1)
Pubmed 4 references available
Quantity 0.1 mg
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Immunogen Purified recombinant fragment of KARS(aa90-174) expressed in E. Coli.
Isotype IgG1
Specificity Purified recombinant fragment of KARS(aa90-174) expressed in E. Coli.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name KARS (KARS Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 68048 DA
Gene ID 12406
NCBI Accession NP_005539
UniProt Q15046
Research Area Translation Factors, Phospho-specific antibodies, Cell Signaling, Cell Structure, Chromatin, Signaling
Application Notes Western Bloting: = 1:500 - 2000.
Comment

Background: KARS: lysyl-tRNA synthetase. Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Sampath, Mazumder, Seshadri et al.: "Noncanonical function of glutamyl-prolyl-tRNA synthetase: gene-specific silencing of translation." in: Cell, Vol. 119, Issue 2, pp. 195-208, 2004 (PubMed).

Sang Lee, Gyu Park, Park et al.: "Interaction network of human aminoacyl-tRNA synthetases and subunits of elongation factor 1 complex." in: Biochemical and biophysical research communications, Vol. 291, Issue 1, pp. 158-64, 2002 (PubMed).

Cen, Khorchid, Javanbakht et al.: "Incorporation of lysyl-tRNA synthetase into human immunodeficiency virus type 1." in: Journal of virology, Vol. 75, Issue 11, pp. 5043-8, 2001 (PubMed).

Tolkunova, Park, Xia et al.: "The human lysyl-tRNA synthetase gene encodes both the cytoplasmic and mitochondrial enzymes by means of an unusual alternative splicing of the primary transcript." in: The Journal of biological chemistry, Vol. 275, Issue 45, pp. 35063-9, 2000 (PubMed).

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Catalog No. ABIN659402
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