Lysyl-tRNA Synthetase (KARS) antibody

Antigen: Lysyl-tRNA Synthetase (KARS)

Synonyms: KRS, KARS2, KIAA0070, CG12141, DmelCG12141, LysRS, AA589550, AL024334, AL033315, AL033367, D8Wsu108e, mKIAA0070, D8Ertd698e, cb530, zgc:92483, kars, MGC53345, krs, kars2, krs-1, MGC52992, KARS, MGC127504, DKFZp468A0216, MGC94484, MGC69375, Tb06.28P18.190

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN659402

Additional Information

Alternative name: KARS

Gene ID: 3735

UniProt: NP_005539.1, Q15046

Immunogen: Purified recombinant fragment of KARS(aa90-174) expressed in E. Coli.

Isotype: IgG1

Description: Other names: KRS, KARS2, KIAA0070

Characteristics: Mouse Monoclonal Antiobody to KARS

Specificity: Purified recombinant fragment of KARS(aa90-174) expressed in E. Coli.

Molecular Weight: 68048 DA

Comments:

Background: KARS: lysyl-tRNA synthetase. Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis.

Synonyms: KRS, KARS2, KIAA0070, CG12141, DmelCG12141, LysRS, AA589550, AL024334, AL033315, AL033367, D8Wsu108e, mKIAA0070, D8Ertd698e, cb530, zgc:92483, kars, MGC53345, krs, kars2, krs-1, MGC52992, KARS, MGC127504, DKFZp468A0216, MGC94484, MGC69375, Tb06.28P18.190

Application Details

Application Notes: The suggested dilution is: ELISA: ~~ 1:10000. Western Bloting: ~~ 1:500 - 2000.

Buffer: Purified recombinant fragment of KARS(aa90-174) expressed in E. Coli.

Storage: KARS Antibody can be stored at 4?, for long term storage, store at -20?.

Research Area: Translation Factors, Phospho-specific antibodies, Cell Signaling, Cell Structure, Chromatin, Signaling

Restrictions: For Research Use only

Publications

Product: Tolkunova, Park, Xia et al.: "The human lysyl-tRNA synthetase gene encodes both the cytoplasmic and mitochondrial enzymes by means of an unusual alternative splicing of the primary transcript." in: The Journal of biological chemistry, Vol. 275, Issue 45, pp. 35063-9, 2000 (PubMed).

Cen, Khorchid, Javanbakht et al.: "Incorporation of lysyl-tRNA synthetase into human immunodeficiency virus type 1." in: Journal of virology, Vol. 75, Issue 11, pp. 5043-8, 2001 (PubMed).

Sang Lee, Gyu Park, Park et al.: "Interaction network of human aminoacyl-tRNA synthetases and subunits of elongation factor 1 complex." in: Biochemical and biophysical research communications, Vol. 291, Issue 1, pp. 158-64, 2002 (PubMed).

Sampath, Mazumder, Seshadri et al.: "Noncanonical function of glutamyl-prolyl-tRNA synthetase: gene-specific silencing of translation." in: Cell, Vol. 119, Issue 2, pp. 195-208, 2004 (PubMed).