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Betaine--Homocysteine S-Methyltransferase (BHMT) antibody

Details for Product No. ABIN659441
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Antigen
Synonyms BHMT1, hm:zehn2153, wu:fb53h01, wu:fb63c08, wu:fj64d01, zgc:123027
Reactivity
Human
(62), (16), (13), (7), (2), (1), (1), (1), (1), (1), (1)
Host
Mouse
(39), (20), (8)
Clonality
Monoclonal
Application
Western Blotting (WB)
(57), (30), (22), (15), (7), (5), (4), (2)
Pubmed 2 references available
Quantity 0.1 mg
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Catalog No. ABIN659441
357.50 $
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Immunogen Purified recombinant fragment of BHMT expressed in E. Coli.
Isotype IgG1
Specificity Purified recombinant fragment of BHMT expressed in E. Coli.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name BHMT
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 44998 DA
Gene ID 12406
UniProt Q93088, NP_001704.2
Research Area Signaling, Cell Structure
Application Notes Western Bloting: = 1:500 - 2000.
Comment

Background: BHMT: betaine-homocysteine methyltransferase. This protein is a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Ou, Yang, Ramani et al.: "Inhibition of human betaine-homocysteine methyltransferase expression by S-adenosylmethionine and methylthioadenosine." in: The Biochemical journal, Vol. 401, Issue 1, pp. 87-96, 2006 (PubMed).

Validation Images
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