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Details for Product No. ABIN659441

Betaine--Homocysteine S-Methyltransferase (BHMT) antibody

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Antigen
Synonyms BHMT1, hm:zehn2153, wu:fb53h01, wu:fb63c08, wu:fj64d01, zgc:123027, bhmt
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality Monoclonal
Application
»Alternatives Western Blotting (WB), ELISA
Pubmed 2 references available
Catalog no. ABIN659441
Quantity 0.1 mg
Price
357.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Immunogen Purified recombinant fragment of BHMT expressed in E. Coli.
Isotype IgG1
Specificity Purified recombinant fragment of BHMT expressed in E. Coli.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name BHMT
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 44998 DA
Gene ID 12406
UniProt Q93088, NP_001704.2
Research Area Signaling, Cell Structure
Application Notes The suggested dilution is: ELISA: ~~ 1:10000. Western Bloting: ~~ 1:500 - 2000.
Comment

Background: BHMT: betaine-homocysteine methyltransferase. This protein is a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Ou, Yang, Ramani et al.: "Inhibition of human betaine-homocysteine methyltransferase expression by S-adenosylmethionine and methylthioadenosine." in: The Biochemical journal, Vol. 401, Issue 1, pp. 87-96, 2006 (PubMed).

Alternatives for antigen "Betaine--Homocysteine S-Methyltransferase (BHMT)", type "Antibodies"
Hosts (36), (18), (9)
Reactivities (58), (14), (11), (8), (2), (2), (1), (1), (1), (1)
Applications (55), (32), (19), (13), (5), (4), (2), (2)
Epitopes (9), (7), (5), (1), (1)
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