V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) antibody

Antigen: V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF)

Synonyms: BRAF1, RAFB1, B-RAF1, FLJ95109, MGC126806, MGC138284, RMIL, C-RMIL, B-Raf, braf1, rafb1, b-raf1, BRAF

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN659471

Additional Information

Alternative name: BRAF

Gene ID: 673

UniProt: P15056, NP_004324.2

Immunogen: Purified recombinant fragment of human BRAF expressed in E. Coli.

Isotype: IgG1

Description: Other names: BRAF1, RAFB1, B-RAF1, FLJ95109

Characteristics: Mouse Monoclonal Antibody to BRAF

Specificity: Purified recombinant fragment of human BRAF expressed in E. Coli.

Molecular Weight: 84437 DA

Comments:

Background: BRAF: v-raf murine sarcoma viral oncogene homolog B1, also known as BRAF1, RAFB1, B-RAF1, FLJ95109. Entrez Protein NP_004324. It is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439). B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK), it has a long amino-terminal region,the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers. The BRAF gene mutation is frequently detected in papillary thyroid carcinoma,melanocytic nevi, primary cutaneous melanomas and colorectal cancers.

Application Details

Application Notes: The suggested dilution is: ELISA: ~~ 1:10000. Western Bloting: ~~ 1:500 - 2000. Immunohistochemistry: ~~ 1:200 - 1000. Immunofluorescence: ~~ 1:200 - 1000.

Buffer: Purified recombinant fragment of human BRAF expressed in E. Coli.

Storage: BRAF Antibody can be stored at 4?, for long term storage, store at -20?.

Research Area: Phospho-specific antibodies, Cell Signaling, Cancer, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Riesco-Eizaguirre, Gutiérrez-Martínez, García-Cabezas et al.: "The oncogene BRAF V600E is associated with a high risk of recurrence and less differentiated papillary thyroid carcinoma due to the impairment of Na+/I- targeting to the membrane." in: Endocrine-related cancer, Vol. 13, Issue 1, pp. 257-69, 2006 (PubMed).

Di Nicolantonio, Martini, Molinari et al.: "Wild-type BRAF is required for response to panitumumab or cetuximab in metastatic colorectal cancer." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 26, Issue 35, pp. 5705-12, 2008 (PubMed).