GATA Binding Protein 4 (GATA4) antibody

Details for Product No. ABIN659508
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Antigen
Reactivity
Human
(159), (102), (92), (2), (2), (1), (1)
Host
Mouse
(134), (25), (5), (2)
Clonality
Monoclonal
Conjugate
Un-conjugated
(3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1)
Application
Western Blotting (WB)
(122), (70), (46), (29), (20), (18), (11), (3), (3), (2), (1), (1), (1)
Pubmed 3 references available
Quantity 0.1 mg
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Immunogen Purified recombinant fragment of human GATA4 expressed in E. Coli.
Isotype IgG2b
Specificity Purified recombinant fragment of human GATA4 expressed in E. Coli.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name GATA4 (GATA4 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 44565 DA
Gene ID 12406
NCBI Accession NP_002043
UniProt P43694
Research Area Translation Factors, Phospho-specific antibodies, Transcription Factors, Signaling, Cell Structure
Application Notes Western Bloting: = 1:500 - 2000.
Comment

Background: GATA binding protein 4, also known as GATA4, it is a 46 kDa member of the GATA family of zinc-finger transcription factors. Members of this family is involved in the development of cardiac hypertrophy and remodeling, and plays a critical role in regulating basal and agonist or stress induced gene expression in cardiac and smooth muscle cell types. These factors recognize the GATA motif which is present in the promoters of many genes. GATA4 contains a conserved MAPK phosphorylation site at serine 105 within the transcriptional activation domain. Serine 105 of GATA4 is phosphorylated in response to agonist stimulation through MEK 1 ERK1 / 2, and weakly through JNK or p38 MAPKs. GATA4 is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiac septal defects.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Rajagopal, Ma, Obler et al.: "Spectrum of heart disease associated with murine and human GATA4 mutation." in: Journal of molecular and cellular cardiology, Vol. 43, Issue 6, pp. 677-85, 2007 (PubMed).

Belaguli, Sepulveda, Nigam et al.: "Cardiac tissue enriched factors serum response factor and GATA-4 are mutual coregulators." in: Molecular and cellular biology, Vol. 20, Issue 20, pp. 7550-8, 2000 (PubMed).

Durocher, Charron, Warren et al.: "The cardiac transcription factors Nkx2-5 and GATA-4 are mutual cofactors." in: The EMBO journal, Vol. 16, Issue 18, pp. 5687-96, 1997 (PubMed).

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Catalog No. ABIN659508
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