GATA Binding Protein 4 (GATA4) antibody

Antigen: GATA Binding Protein 4 (GATA4)

Synonyms: MGC126629, Gata-4, GATA-4, GATA4, gata4, gata-4, xgata4, xGATA-4, MGC81427, GATA transcription factor 4, Gata4

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN659508

Additional Information

Alternative name: GATA4

Gene ID: 2626

UniProt: NP_002043.2, P43694

Immunogen: Purified recombinant fragment of human GATA4 expressed in E. Coli.

Isotype: IgG2b

Description: Other names: MGC126629

Characteristics: Mouse Monoclonal Antibody to GATA4

Specificity: Purified recombinant fragment of human GATA4 expressed in E. Coli.

Molecular Weight: 44565 DA

Comments:

Background: GATA binding protein 4, also known as GATA4, it is a 46 kDa member of the GATA family of zinc-finger transcription factors. Members of this family is involved in the development of cardiac hypertrophy and remodeling, and plays a critical role in regulating basal and agonist or stress induced gene expression in cardiac and smooth muscle cell types. These factors recognize the GATA motif which is present in the promoters of many genes. GATA4 contains a conserved MAPK phosphorylation site at serine 105 within the transcriptional activation domain. Serine 105 of GATA4 is phosphorylated in response to agonist stimulation through MEK 1 ERK1 / 2, and weakly through JNK or p38 MAPKs. GATA4 is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiac septal defects.

Application Details

Application Notes: The suggested dilution is: ELISA: ~~ 1:10000. Western Bloting: ~~ 1:500 - 2000.

Buffer: Purified recombinant fragment of human GATA4 expressed in E. Coli.

Storage: GATA4 Antibody can be stored at 4?, for long term storage, store at -20?.

Research Area: Translation Factors, Phospho-specific antibodies, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Publications

Product: Durocher, Charron, Warren et al.: "The cardiac transcription factors Nkx2-5 and GATA-4 are mutual cofactors." in: The EMBO journal, Vol. 16, Issue 18, pp. 5687-96, 1997 (PubMed).

Belaguli, Sepulveda, Nigam et al.: "Cardiac tissue enriched factors serum response factor and GATA-4 are mutual coregulators." in: Molecular and cellular biology, Vol. 20, Issue 20, pp. 7550-8, 2000 (PubMed).

Rajagopal, Ma, Obler et al.: "Spectrum of heart disease associated with murine and human GATA4 mutation." in: Journal of molecular and cellular cardiology, Vol. 43, Issue 6, pp. 677-85, 2007 (PubMed).