Wingless-Type MMTV Integration Site Family, Member 1 (WNT1) antibody

Antigen: Wingless-Type MMTV Integration Site Family, Member 1 (WNT1)

Synonyms: INT1, wnt1-a, Xint-1

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN659520

Additional Information

Alternative name: WNT1

Gene ID: 7471

UniProt: NP_005421.1, P04628

Immunogen: Purified recombinant fragment of WNT1 expressed in E. Coli.

Isotype: IgG1

Description: Other names: INT1

Characteristics: Mouse Monoclonal Antibody to WNT1

Specificity: Purified recombinant fragment of WNT1 expressed in E. Coli.

Molecular Weight: 40982 DA

Comments:

Background: WNT1: wingless-type MMTV integration site family, member 1. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant rolein Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region.

Application Details

Application Notes: The suggested dilution is: ELISA: ~~ 1:10000. Western Bloting: ~~ 1:500 - 2000. Immunofluorescence: ~~ 1:200 - 1000. Flow cytometry: ~~ 1:200 - 400.

Buffer: Purified recombinant fragment of WNT1 expressed in E. Coli.

Storage: WNT1 Antibody can be stored at 4?, for long term storage, store at -20?.

Research Area: Cell Signaling, Cell Structure

Restrictions: For Research Use only

Publications

Product: Mikami, You, He et al.: "Efficacy of Wnt-1 monoclonal antibody in sarcoma cells." in: BMC cancer, Vol. 5, pp. 53, 2005 (PubMed).

Woll, Morris, Painschab et al.: "Wnt signaling promotes hematoendothelial cell development from human embryonic stem cells." in: Blood, Vol. 111, Issue 1, pp. 122-31, 2007 (PubMed).