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p65 antibody (Nuclear Factor-KB P65)

Details for Product anti-NFkBP65 Antibody No. ABIN659539, Supplier: Login to see New
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Antigen
Alternatives
Reactivity
Human
(1070), (484), (398), (43), (37), (26), (14), (10), (6), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host
Mouse
(996), (81), (13), (8), (2)
Clonality
Monoclonal
Conjugate
This p65 antibody is un-conjugated
(31), (30), (22), (18), (16), (16), (11), (9), (9), (8), (8), (8), (8), (8), (8), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(785), (408), (338), (177), (169), (134), (90), (44), (30), (24), (21), (20), (11), (10), (7), (7), (7), (4), (2), (2), (1), (1)
Pubmed 3 references available
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Quantity 0.1 mg
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Immunogen Purified recombinant fragment of human NF-?B p65 expressed in E. Coli.
Isotype IgG1
Specificity Purified recombinant fragment of human NF-?B p65 expressed in E. Coli.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name NF-kB p65 (NFkBP65 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 60219 DA
Gene ID 12406
NCBI Accession NP_068810
UniProt Q04206
Research Area Ubiquitin-related antibodies, Translation Factors, Phospho-specific antibodies, Transcription Factors, Signaling, Cell Structure
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Western Bloting: = 1:500 - 2000.
Comment

Background: Transcription factors of the nuclear factor ? B (NF-?B)/Rel family is a ubiquitously expressed transcription factor that regulates many cytokine and Ig genes. It is involved in immune, inflammatory, viral, and acute phase responses. There are five family members in mammals: RelA (p65), c-Rel, RelB, NF-?B1 (p105/p50) and NF-?B2 (p100/p52). The most studied NF-?B complex consists of the p50 and p65 subunits, both containing a 300 amino acid region with homology to the Rel proto-oncogene product. The p50 subunit binds DNA, whereas the p65 subunit is responsible for the interaction of NF-?B with its inhibitor, I?B. In most cell types, the p50/p65 heterodimer is located within the cytoplasm complexed to I?B. This complex prevents nuclear translocation and activity of NF-?B. In response to stimuli such as cytokines, LPS, and viral infections, I?B is phosphorylated at critical residues. This phosphorylation induces dissociation of the I?B/NF-?B complex, allowing the free heterodimeric NF-?B to form a heterotetramer that translocates to the nucleus. In the nucleus, it binds to the ?B site within promoters and enhancers and functions as a transcriptional activator.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Yamit-Hezi, Nir, Wolstein et al.: "Interaction of TAFII105 with selected p65/RelA dimers is associated with activation of subset of NF-kappa B genes." in: The Journal of biological chemistry, Vol. 275, Issue 24, pp. 18180-7, 2000 (PubMed).

Jacobs, Harrison: "Structure of an IkappaBalpha/NF-kappaB complex." in: Cell, Vol. 95, Issue 6, pp. 749-58, 1999 (PubMed).

DiDonato, Hayakawa, Rothwarf et al.: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997 (PubMed).

Catalog No. ABIN659539

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