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Epidermal Growth Factor (EGF) antibody

Antigen

Epidermal Growth Factor (EGF)
Synonyms EGF, CEGF, LOC100218747, URG, HOMG4, AI790464
Clonality Monoclonal
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
4 references available
Certificates ISO 9001:2008
Catalog no. ABIN659547
Quantity 0.1ml  (Variants)
Price 357.50 $   Plus shipping costs $45.00
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Additional Information
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Alternative name EGF
Gene ID 1950
UniProt NP_001954.2, P01133
Immunogen Purified recombinant fragment of human EGF expressed in E. Coli.
Isotype IgG1
Description Other names: URG, HOMG4, EGF
Characteristics Mouse Monoclonal Antibody to EGF
Specificity Purified recombinant fragment of human EGF expressed in E. Coli.
Molecular Weight 133994 DA
Comments

Background: Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.

Application Details
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Application Notes The suggested dilution is: ELISA: ~~ 1:10000. Western Bloting: ~~ 1:500 - 2000. Immunohistochemistry: ~~ 1:200 - 1000.
Buffer Purified recombinant fragment of human EGF expressed in E. Coli.
Storage EGF Antibody can be stored at 4?, for long term storage, store at -20?.
Research Area Cell Signaling, Cell Structure
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Product Gout, Dhand, Panayotou et al.: "Expression and characterization of the p85 subunit of the phosphatidylinositol 3-kinase complex and a related p85 beta protein by using the baculovirus expression system." in: The Biochemical journal, Vol. 288 ( Pt 2), pp. 395-405, 1993 (PubMed).

Gual, Giordano, Williams et al.: "Sustained recruitment of phospholipase C-gamma to Gab1 is required for HGF-induced branching tubulogenesis." in: Oncogene, Vol. 19, Issue 12, pp. 1509-18, 2000 (PubMed).

Soubeyran, Kowanetz, Szymkiewicz et al.: "Cbl-CIN85-endophilin complex mediates ligand-induced downregulation of EGF receptors." in: Nature, Vol. 416, Issue 6877, pp. 183-7, 2002 (PubMed).

Yacoub, McKinstry, Hinman et al.: "Epidermal growth factor and ionizing radiation up-regulate the DNA repair genes XRCC1 and ERCC1 in DU145 and LNCaP prostate carcinoma through MAPK signaling." in: Radiation research, Vol. 159, Issue 4, pp. 439-52, 2003 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Epidermal Growth Factor (EGF)", type "Antibodies"
Hosts Rabbit (166), Mouse (86), Goat (17), Rat (3), Sheep (3), Chicken (1)
Reactivities Human (144), Mouse (Murine) (89), Rat (Rattus) (68), Pig (Porcine) (4), Wild boar (Sus scrofa) (4), Cow (Bovine) (3), Dog (Canine) (3), Sponges (Suberites domuncula) (1)
Applications ELISA (158), Western Blotting (WB) (133), Immunofluorescence (IF) (97), Flow Cytometry (FACS) (46), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (32), Immunohistochemistry (IHC) (27), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (18), Neutralization (Neut) (14), Immunohistochemistry (Frozen Sections) (IHC (fro)) (9), Immunocytochemistry (ICC) (8), Functional Studies (Func) (7), Immunoprecipitation (IP) (7), Immunoelectron Microscopy (IEM) (6), Blocking Antibody (Inhibition) (4), Blocking Antibody (Inhibition) (4), Radioimmunoassay (RIA) (3), Dot Blot (DB) (2), ELISA (Capture) (2), ELISA (Detection) (2), Enzyme Immunoassay (EIA) (2), Immunohistochemistry (Fixed) (IHC (fx)) (2)
Conjugates Biotin (27), HRP (10), FITC (8), Alexa Fluor 350 (6), Alexa Fluor 488 (6), Alexa Fluor 555 (6), Alexa Fluor 647 (6), Cy3 (6), Cy5 (6), Cy5.5 (6), Cy7 (6), Gold (6), PE (6), PE,Cy3 (3), PE,Cy5 (3), PE,Cy5.5 (3), PE,Cy7 (3), PE-Cy3 (3), PE-Cy5 (3), PE-Cy5.5 (3), PE-Cy7 (3)
Epitopes C-Term (54), Internal Region (5), Center (2), Extracellular Domain (2), AA 648-1186 (1), C-Term,AA 939-967 (1), Internal Region,AA 689-720 (1), pTyr1068 (1), pTyr1173 (1)