anti-EGF antibody (Epidermal Growth Factor)

Details for Product anti-EGF Antibody No. ABIN659547
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Antigen
Reactivity
Human
(305), (101), (72), (14), (12), (11), (7), (3), (3), (1)
Host
Mouse
(290), (143), (31), (11), (7), (3)
Clonality
Monoclonal
Conjugate
This EGF antibody is un-conjugated
(57), (28), (24), (10), (10), (10), (6), (6), (6), (6), (6), (6), (6), (6)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(327), (297), (86), (65), (60), (33), (31), (30), (29), (18), (14), (12), (10), (10), (7), (5), (4), (2), (2), (2), (2), (1)
Pubmed 4 references available
Quantity 0.1 mg
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Immunogen Purified recombinant fragment of human EGF expressed in E. Coli.
Isotype IgG1
Specificity Purified recombinant fragment of human EGF expressed in E. Coli.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name EGF (EGF Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 133994 DA
Gene ID 12406
NCBI Accession NP_001954
UniProt P01133
Research Area Cell Signaling, Cell Structure
Application Notes Western Bloting: = 1:500 - 2000, Immunohistochemistry: = 1:200 - 1000.
Comment

Background: Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Yacoub, McKinstry, Hinman et al.: "Epidermal growth factor and ionizing radiation up-regulate the DNA repair genes XRCC1 and ERCC1 in DU145 and LNCaP prostate carcinoma through MAPK signaling." in: Radiation research, Vol. 159, Issue 4, pp. 439-52, 2003 (PubMed).

Soubeyran, Kowanetz, Szymkiewicz et al.: "Cbl-CIN85-endophilin complex mediates ligand-induced downregulation of EGF receptors." in: Nature, Vol. 416, Issue 6877, pp. 183-7, 2002 (PubMed).

Gual, Giordano, Williams et al.: "Sustained recruitment of phospholipase C-gamma to Gab1 is required for HGF-induced branching tubulogenesis." in: Oncogene, Vol. 19, Issue 12, pp. 1509-18, 2000 (PubMed).

Gout, Dhand, Panayotou et al.: "Expression and characterization of the p85 subunit of the phosphatidylinositol 3-kinase complex and a related p85 beta protein by using the baculovirus expression system." in: The Biochemical journal, Vol. 288 ( Pt 2), pp. 395-405, 1993 (PubMed).

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Catalog No. ABIN659547
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