anti-NGFR antibody (Nerve Growth Factor Receptor)

Details for Product anti-NGFR Antibody No. ABIN659550
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Antigen
Reactivity
Human
(206), (93), (83), (7), (7), (5), (5), (4), (4), (3), (3), (1), (1), (1), (1), (1), (1)
Host
Mouse
(134), (66), (34), (4), (1)
Clonality
Monoclonal
Conjugate
This NGFR antibody is un-conjugated
(15), (12), (9), (8), (6), (4), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
(179), (88), (66), (50), (47), (30), (26), (25), (20), (17), (10), (6), (6), (4), (4), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
Quantity 0.1 mg
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Immunogen Purified recombinant fragment of human NGFR expressed in E. Coli.
Isotype IgG1
Specificity Purified recombinant fragment of human NGFR expressed in E. Coli.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name NGFR (NGFR Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 45183 DA
Gene ID 12406
NCBI Accession NP_002498
UniProt P08138
Research Area Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Cell Structure, Neurology
Application Notes Western Bloting: = 1:500 - 2000. Immunofluorescence: = 1:200 - 1000. Flow cytometry: = 1:200 - 400.
Comment

Background: Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGFR p75 plays a central role in the regulation of cell number by apoptosis in the developing CNS. During early development, activation of NGFR p75 by NGF induces apoptotic cell death in some neuronal cells, probably through activation of the sphingomyelinase/ceramide pathway, the ICE like proteases and the JNK pathway. In rat Schwann cells, NGF binding to NGFR p75 activates NF kappaB, possibly to modulate Schwann cell migration during nerve regeneration. CD271 has recently been described as being expressed in mesenchymal stem cells (bone marrow stromal cells).

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications He, Garcia: "Structure of nerve growth factor complexed with the shared neurotrophin receptor p75." in: Science (New York, N.Y.), Vol. 304, Issue 5672, pp. 870-5, 2004 (PubMed).

Hu, Zhang, Qin et al.: "Increased p75(NTR) expression in hippocampal neurons containing hyperphosphorylated tau in Alzheimer patients." in: Experimental neurology, Vol. 178, Issue 1, pp. 104-11, 2002 (PubMed).

DiDonato, Hayakawa, Rothwarf et al.: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997 (PubMed).

Fantini, Johansson: "Expression of growth-associated protein 43 and nerve growth factor receptor in human skin: a comparative immunohistochemical investigation." in: The Journal of investigative dermatology, Vol. 99, Issue 6, pp. 734-42, 1993 (PubMed).

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Catalog No. ABIN659550
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