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Transcription Factor 3 (E2A Immunoglobulin Enhancer Binding Factors E12/E47) (TCF3) antibody

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(132), (51), (50), (5), (4), (2), (1), (1), (1)
(95), (20), (19), (2)
(4), (4), (4), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Flow Cytometry (FACS), Western Blotting (WB)
(103), (74), (55), (24), (14), (13), (3), (3), (2)
Pubmed 3 references available
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Quantity 0.1 mg
Shipping to United States ( )
Immunogen Purified recombinant fragment of human TCF3 expressed in E. Coli.
Isotype IgG1
Specificity Purified recombinant fragment of human TCF3 expressed in E. Coli.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name TCF3 (TCF3 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 67600 DA
Gene ID 12406
NCBI Accession NP_003191
UniProt P15923
Research Area Phospho-specific antibodies, Transcription Factors, Chromatin, Signaling, Cell Structure
Application Notes The suggested dilution is: Western Bloting: = 1:500 - 2000. Flow cytometry: = 1:200 - 400.

Background: Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Bradney, Hjelmeland, Komatsu et al.: "Regulation of E2A activities by histone acetyltransferases in B lymphocyte development." in: The Journal of biological chemistry, Vol. 278, Issue 4, pp. 2370-6, 2003 (PubMed).

Snider, Thirlwell, Miller et al.: "Inhibition of Tcf3 binding by I-mfa domain proteins." in: Molecular and cellular biology, Vol. 21, Issue 5, pp. 1866-73, 2001 (PubMed).

Firulli, Hadzic, McDaid et al.: "The basic helix-loop-helix transcription factors dHAND and eHAND exhibit dimerization characteristics that suggest complex regulation of function." in: The Journal of biological chemistry, Vol. 275, Issue 43, pp. 33567-73, 2000 (PubMed).

Catalog No. ABIN659566

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