Transcription Factor 3 (E2A Immunoglobulin Enhancer Binding Factors E12/E47) (TCF3) antibody

Details for Product No. ABIN659566
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Antigen
Synonyms Pan2, Tcfe2a, E2A, E47, ITF1, TCF-3, VDIR, bHLHb21, A1, AA408400, ALF2, AW209082, E12, E12/E47, ME2, Pan1, bhlhb21, e2a, itf1, tcfe2a, vdir, xtcf-3, xtcf3, G12, TCF7L1, tcf3a, zgc:113045
Reactivity
Human
(102), (70), (57), (26), (26), (25), (12), (2), (1)
Host
Mouse
(85), (12), (7), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application
Western Blotting (WB), Flow Cytometry (FACS)
(65), (55), (30), (29), (17), (9), (1)
Pubmed 3 references available
Catalog no. ABIN659566
Quantity 0.1 mg
Price
357.50 $   Plus shipping costs $45.00
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Immunogen Purified recombinant fragment of human TCF3 expressed in E. Coli.
Isotype IgG1
Specificity Purified recombinant fragment of human TCF3 expressed in E. Coli.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name TCF3
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 67600 DA
Gene ID 12406
UniProt NP_003191.1, P15923
Research Area Phospho-specific antibodies, Transcription Factors, Chromatin, Signaling, Cell Structure
Application Notes The suggested dilution is: Western Bloting: = 1:500 - 2000. Flow cytometry: = 1:200 - 400.
Comment

Background: Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Firulli, Hadzic, McDaid et al.: "The basic helix-loop-helix transcription factors dHAND and eHAND exhibit dimerization characteristics that suggest complex regulation of function." in: The Journal of biological chemistry, Vol. 275, Issue 43, pp. 33567-73, 2000 (PubMed).

Snider, Thirlwell, Miller et al.: "Inhibition of Tcf3 binding by I-mfa domain proteins." in: Molecular and cellular biology, Vol. 21, Issue 5, pp. 1866-73, 2001 (PubMed).

Bradney, Hjelmeland, Komatsu et al.: "Regulation of E2A activities by histone acetyltransferases in B lymphocyte development." in: The Journal of biological chemistry, Vol. 278, Issue 4, pp. 2370-6, 2003 (PubMed).

Hosts (85), (12), (7), (1)
Reactivities (102), (70), (57), (26), (26), (25), (12), (2), (1)
Applications (65), (55), (30), (29), (17), (9), (1)
Conjugates (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Epitopes (21), (12), (10), (9), (7), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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