p44/42 (MAPK) (Erk1/2) antibody

Antigen: p44/42 (MAPK) (Erk1/2)

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN659568

Additional Information

Gene ID: 5594

UniProt: P28482, NP_620407.1

Immunogen: Purified recombinant fragment of human MAPK1 expressed in E. Coli.

Isotype: IgG2b

Description: Other names: ERK, p38, p40, p41, ERK2, ERT1, MAPK2, PRKM1, PRKM2, P42MAPK, p41mapk, MAPK1

Characteristics: Mouse Monoclonal Antibody to p44/42 MAPK (Erk1/2)

Specificity: Purified recombinant fragment of human MAPK1 expressed in E. Coli.

Molecular Weight: 41390 DA

Comments:

Background: Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines and is an important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors such as U0126 and PD98059.

Application Details

Application Notes: The suggested dilution is: ELISA: ~~ 1:10000. Western Bloting: ~~ 1:500 - 2000. Flow cytometry: ~~ 1:200 - 400.

Buffer: Purified recombinant fragment of human MAPK1 expressed in E. Coli.

Storage: p44/42 (MAPK) (Erk1/2) Antibody can be stored at 4?, for long term storage, store at -20?.

Research Area: Translation Factors, Phospho-specific antibodies, Cell Signaling, Transcription Factors, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Gonzalez, Raden, Rigby et al.: "Heterogeneous expression of four MAP kinase isoforms in human tissues." in: FEBS letters, Vol. 304, Issue 2-3, pp. 170-8, 1992 (PubMed).

Schiemann, Graves, Baumann et al.: "Phosphorylation of the human leukemia inhibitory factor (LIF) receptor by mitogen-activated protein kinase and the regulation of LIF receptor function by heterologous receptor activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 12, pp. 5361-5, 1995 (PubMed).

Lønne, Masoumi, Lennartsson et al.: "Protein kinase Cdelta supports survival of MDA-MB-231 breast cancer cells by suppressing the ERK1/2 pathway." in: The Journal of biological chemistry, Vol. 284, Issue 48, pp. 33456-65, 2009 (PubMed).

Tabakoff, Saba, Printz et al.: "Genetical genomic determinants of alcohol consumption in rats and humans." in: BMC biology, Vol. 7, pp. 70, 2009 (PubMed).