Did you know that you can buy products from over 140 different suppliers from us?

p44/42 MAPK (Erk1/2) antibody

Details for Product No. ABIN659568
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Reactivity
Human
(2), (2), (1), (1), (1)
Host
Mouse
(1), (1)
Clonality
Monoclonal
Application
Western Blotting (WB), Flow Cytometry (FACS)
(2), (1), (1), (1)
Pubmed 4 references available
Quantity 0.1 mg
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days
Catalog No. ABIN659568
357.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Purified recombinant fragment of human MAPK1 expressed in E. Coli.
Isotype IgG2b
Specificity Purified recombinant fragment of human MAPK1 expressed in E. Coli.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 41390 DA
Gene ID 12406
UniProt P28482, NP_620407.1
Research Area Translation Factors, Phospho-specific antibodies, Cell Signaling, Transcription Factors, Signaling, Chromatin, Cell Structure
Application Notes The suggested dilution is: Western Bloting: = 1:500 - 2000. Flow cytometry: = 1:200 - 400.
Comment

Background: Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines and is an important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors such as U0126 and PD98059.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Gonzalez, Raden, Rigby et al.: "Heterogeneous expression of four MAP kinase isoforms in human tissues." in: FEBS letters, Vol. 304, Issue 2-3, pp. 170-8, 1992 (PubMed).

Schiemann, Graves, Baumann et al.: "Phosphorylation of the human leukemia inhibitory factor (LIF) receptor by mitogen-activated protein kinase and the regulation of LIF receptor function by heterologous receptor activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 12, pp. 5361-5, 1995 (PubMed).

Lønne, Masoumi, Lennartsson et al.: "Protein kinase Cdelta supports survival of MDA-MB-231 breast cancer cells by suppressing the ERK1/2 pathway." in: The Journal of biological chemistry, Vol. 284, Issue 48, pp. 33456-65, 2009 (PubMed).

Tabakoff, Saba, Printz et al.: "Genetical genomic determinants of alcohol consumption in rats and humans." in: BMC biology, Vol. 7, pp. 70, 2009 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN659568
357.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
Did you look for something else?
back to top