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Details for Product No. ABIN659594

Cyclin-Dependent Kinase 1 (CDK1) antibody

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Antigen
Synonyms
CDC2, CDC28A, P34CDC2, Cdc2, Cdc2a, p34, cdc2, wu:fc30e01, zgc:92032, 5363, CDCDm, CDK1, CDK1/CDC2, CG5363, Cdk-1, Cdk1, Dcdc2, Dm cdc2, DmCdc2, DmCdk1, Dmcdc2, Dmel\\CG5363, cdc, cdc2Dm, cdk1,  ... show more
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality Monoclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), ELISA
Pubmed 2 references available
Catalog no. ABIN659594
Quantity 0.1 mg
Price
357.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Immunogen Purified recombinant fragment of CDC2 expressed in E. Coli.
Isotype IgG1
Specificity Purified recombinant fragment of CDC2 expressed in E. Coli.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name CDC2
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 34081 DA
Gene ID 12406
UniProt P06493, NP_203698.1
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Cell Cycle, Signaling, Chromatin, Cell Structure
Application Notes The suggested dilution is: ELISA: ~~ 1:10000. Western Bloting: ~~ 1:500 - 2000. Flow cytometry: ~~ 1:200 - 400.
Comment

Background: The cell division control protein cdc2, also known as cyclin-dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S-phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Tang, Wu, Guo et al.: "Cdc2 and Mos regulate Emi2 stability to promote the meiosis I-meiosis II transition." in: Molecular biology of the cell, Vol. 19, Issue 8, pp. 3536-43, 2008 (PubMed).

Meyer, Merkel, Brückl et al.: "Cdc2 as prognostic marker in stage UICC II colon carcinomas." in: European journal of cancer (Oxford, England : 1990), Vol. 45, Issue 8, pp. 1466-73, 2009 (PubMed).

Alternatives for antigen "Cyclin-Dependent Kinase 1 (CDK1)", type "Antibodies"
Hosts (338), (128)
Reactivities (420), (248), (236), (45), (38), (31), (27), (26), (14), (9), (7), (4), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applications (376), (157), (129), (84), (73), (60), (53), (32), (15), (11), (11), (10), (7), (4), (3), (3), (1), (1), (1), (1)
Conjugates (13), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (1), (1)
Epitopes (50), (49), (45), (24), (17), (15), (10), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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