BMX Non-Receptor Tyrosine Kinase (BMX) antibody

Antigen: BMX Non-Receptor Tyrosine Kinase (BMX)

Synonyms: ETK, PSCTK2, PSCTK3, Etk, Tyro8, Etk/Bmx, RGD1565643, BMX

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN659597

Additional Information

Alternative name: BMX

Gene ID: 660

UniProt: NP_975010.1, P51813

Immunogen: Purified recombinant fragment of human BMX expressed in E. Coli.

Isotype: IgG1

Description: Other names: ETK, PSCTK2, PSCTK3, BMX

Characteristics: Mouse Monoclonal Antibody to BMX

Specificity: Purified recombinant fragment of human BMX expressed in E. Coli.

Molecular Weight: 78011 DA

Comments:

Background: BMX (bone marrow X kinase) is a cytoplasmic tyrosine kinase identified by reverse transcription of mRNA isolated from human bone marrow and mapped to the chromosomal band Xp22.2. The full length protein is 675 amino acids with a tyrosine kinase domain, an amino terminal pleckstrin domain, as well as an SH3 and SH2 domain. Direct comparison of BMX's primary sequence with other kinases showed that this is highly related to the family of BTK/ITK/TEC. BMX kinase is expressed in fetal and adult tissues, with the highest expression in heart, testis, small intestine and colon. It is undetectable in spleen, brain, kidney, and pancreas. Further analysis of mRNA expression showed that BMX is expressed in hematopoietic tissues and neutrophilic granulocytes, and in patients with acute and myeloid leukemia. The levels of BMX mRNA were substantially lower in patients with acute and chronic lymphoid leukemias, thus suggesting that BMX may be important during myelopoiesis.CST:It is expressed in a variety of hematopoietic, epithelial and endothelial cells.

Application Details

Application Notes: The suggested dilution is: ELISA: ~~ 1:10000. Western Bloting: ~~ 1:500 - 2000.

Buffer: Purified recombinant fragment of human BMX expressed in E. Coli.

Storage: BMX Antibody can be stored at 4?, for long term storage, store at -20?.

Research Area: Ubiquitin-related antibodies, Phospho-specific antibodies, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Kim, Jiang, Xie et al.: "Synergism of cytoplasmic kinases in IL6-induced ligand-independent activation of androgen receptor in prostate cancer cells." in: Oncogene, Vol. 23, Issue 10, pp. 1838-44, 2004 (PubMed).

C�té, Motoyama, Bush et al.: "A novel and evolutionarily conserved PtdIns(3,4,5)P3-binding domain is necessary for DOCK180 signalling." in: Nature cell biology, Vol. 7, Issue 8, pp. 797-807, 2005 (PubMed).

Palmer, Mutch, Workman et al.: "Bmx tyrosine kinase regulates TLR4-induced IL-6 production in human macrophages independently of p38 MAPK and NFkapp}B activity." in: Blood, Vol. 111, Issue 4, pp. 1781-8, 2008 (PubMed).

Chakrabarti, Davidge: "Estradiol modulates tumor necrosis factor-induced endothelial inflammation: role of tumor necrosis factor receptor 2." in: Journal of vascular research, Vol. 50, Issue 1, pp. 21-34, 2012 (PubMed).