Activating Transcription Factor 1 (AFT1) antibody (Biotin)

Antigen: Activating Transcription Factor 1 (AFT1)

Synonyms: TREB36, EWS-ATF1, FUS/ATF-1, Atf-1, zgc:64099, wu:fc92c07, treb36, MGC89098, ews-atf1, fus/atf-1, ATF1, MGC143110

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Conjugate: Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Flow Cytometry (FACS), Immunofluorescence (IF)

Catalog no.: ABIN669533

Additional Information

Alternative name: ATF1

Immunogen: Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from human ATF1 C-terminus

Format: Lyophilized

Reconstitution: Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)

Isotype: IgG

Description: Synonym: Activating Transcription Factor1, TREB36 protein, ATF 1, ATF1 EWS fusion gene ATF1 FUS fusion gene, cAMP dependent transcription factor 1, Cyclic AMP dependent transcription factor ATF 1, Cyclic AMP dependent transcription factor ATF1, EWS AFT1, FUS ATF 1, RNA binding protein activating transcription factor 1 fusion protein, TREB 36, TREB36.

Molecular Weight: 43kDa

Comments:

ATF1 (Activating Transcription Factor 1

TREB-36) is a member of the ATF/CREB family of basic region leucine-zipper (bZip) DNA-binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation

and in stress and immune responses. The activation function of CRE-binding proteins may be modulated by phosphorylation of several kinases and is mediated by coactivators such as CREB-binding protein (CBP) and p300. ATF1 is a nuclear protein that binds DNA as a homodimer or as heterodimers with the inducible transcription factors CREB1 or CREM. Heterodimers appear to be stronger transcriptional activators than the homodimers. Tissue expression of ATF1 mRNA is widespread. Several isoforms of ATF1 arise by differential splicing. ATF1 mediates both Ca2+ and cAMP responses at several levels. It binds to the Tax-responsive element (TRE1) of the human T-cell lymphotropic virus type-I (HTLV1). ATF1 is detectable in metastatic melanoma cells and seems to contribute to their survival. A chimeric protein composed of the N-terminal domain of EWS (Ewing sarcoma oncogene) linked to the bZip domain of ATF1 is implicated in the rare malignant clear cell sarcoma of tendon sheath and aponeuroses (malignant melanoma of soft parts).

Application Details

Application Notes: WB (1:100-500), ELISA (1:500-1000), IP (1:20-100), IHC-P (1:100-500), IHC-F (1:100-500), Flow-Cyt (1:20-100), IF (1:50-200)

Concentration: 1mg/1ml

Purification: Antibodies are purified by protein A and peptide affinity chromatography.

Buffer: 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

Storage: Ship at room temperature. Store at -20°C

Research Area: Chromatin and Nuclear Signaling, Transcription Factors

Restrictions: For Research Use only