ICAM1 antibody (AA 201-300)

Catalog No. ABIN670806

Product Details anti-ICAM1 Antibody

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Binding Specificity: AA 201-300

Reactivity: Human, Mouse, Rat, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ICAM1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Specificity: KO-Validated

Cross-Reactivity: Human, Mouse, Rabbit, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse ICAM1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-ICAM1 antibody (ABIN670806)

Zhang, Hua, Hou, Du, He, Liu, Hu, Yan: "Sphingomyelin synthase 2 promotes H2O2-induced endothelial dysfunction by activating the Wnt/β-catenin signaling pathway." in: International journal of molecular medicine, Vol. 42, Issue 6, pp. 3344-3354, (2019) (PubMed).

Yang, Cheng, Liu, Liu, Tan, Guan, Guo, Kuang: "Datura Metel L. Ameliorates Imiquimod-Induced Psoriasis-Like Dermatitis and Inhibits Inflammatory Cytokines Production through TLR7/8-MyD88-NF-κB-NLRP3 Inflammasome Pathway." in: Molecules (Basel, Switzerland), Vol. 24, Issue 11, (2019) (PubMed).

Kim, Ko, Lee, Kim, Jo, Cho: "Blocking junctional adhesion molecule C promotes the recovery of cisplatin-induced acute kidney injury." in: The Korean journal of internal medicine, Vol. 32, Issue 6, pp. 1053-1061, (2018) (PubMed).

Wang, Li, Liu, Wang, Cao: "Inhibitory effect of Zanthoxylum bungeanum seed oil on ovalbumin‑induced lung inflammation in a murine model of asthma." in: Molecular medicine reports, Vol. 13, Issue 5, pp. 4289-302, (2017) (PubMed).

Tang, Ni, Cai, Ding, Liu, Zhang et al.: "Renoprotective effects of berberine and its potential effect on the expression of β-arrestins and intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 in streptozocin-diabetic ..." in: Journal of diabetes, Vol. 8, Issue 5, pp. 693-700, (2016) (PubMed).

Ji, Wang, Li, Qian: "Up-regulation of ICAM-1mRNA and IL-1βmRNA in lung tissues of a rat model of COPD." in: International journal of clinical and experimental medicine, Vol. 8, Issue 11, pp. 21956-63, (2016) (PubMed).

Ji, Wang, Wang, Chen, Li: "Protective effect of Xuebijing injection against acute lung injury induced by left ventricular ischemia/reperfusion in rabbits." in: Experimental and therapeutic medicine, Vol. 12, Issue 1, pp. 51-58, (2016) (PubMed).

Osada-Oka, Shiota, Izumi, Nishiyama, Tanaka, Yamaguchi, Sakurai, Miura, Iwao: "Macrophage-derived exosomes induce inflammatory factors in endothelial cells under hypertensive conditions." in: Hypertension research : official journal of the Japanese Society of Hypertension, Vol. 40, Issue 4, pp. 353-360, (2016) (PubMed).

Xiong, Xu, Tan, Han, Tang, Xu, Zeng, Guo: "Glycyrrhizin Ameliorates Imiquimod-Induced Psoriasis-like Skin Lesions in BALB/c Mice and Inhibits TNF-?-Induced ICAM-1 Expression via NF-?B/MAPK in HaCaT Cells." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 35, Issue 4, pp. 1335-46, (2015) (PubMed).

Chenna Narendra, Chalise, Magnusson, Uppugunduri: "Local but Not Systemic Administration of Uridine Prevents Development of Antigen-Induced Arthritis." in: PLoS ONE, Vol. 10, Issue 10, pp. e0141863, (2015) (PubMed).

Park, Regmi, Park, Lee, Chang, Ku, Kim, Kim: "Protective effect of 7-O-succinyl macrolactin A against intestinal inflammation is mediated through PI3-kinase/Akt/mTOR and NF-?B signaling pathways." in: European journal of pharmacology, Vol. 735, pp. 184-92, (2014) (PubMed).

Qiao, Li, Li, Zhu, Huang, Shao, Bai: "Intermedin protects against renal ischemia-reperfusion injury by inhibition of oxidative stress." in: American journal of physiology. Renal physiology, Vol. 304, Issue 1, pp. F112-9, (2013) (PubMed).

Chu, He, Kuang, Ren, Gou: "Celastrol protects kidney against ischemia-reperfusion-induced injury in rats." in: The Journal of surgical research, Vol. 186, Issue 1, pp. 398-407, (2013) (PubMed).

Zhang, Lv, Chen, Zou, Lin, Yang, Guo, Lin: "Effect of mild hypothermia on breast cancer cells adhesion and migration." in: Bioscience trends, Vol. 6, Issue 6, pp. 313-24, (2013) (PubMed).

Wang, Shi, Cui, Bai, He, Shang, Su, Xin, Jing, Zhu: "Preliminary Research of Relationship between Acute Peritonitis and Celiac Primo Vessels." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2013, pp. 569161, (2013) (PubMed).

Target Details for ICAM1

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Alternative Name: ICAM1 (ICAM1 Products)

Target Type: Viral Protein

Background:

Synonyms: CD54, Ly-47, Icam-1, MALA-2, Intercellular adhesion molecule 1, MyD1, Icam1

Background: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity).

Gene ID: 15894

UniProt: P13597

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling