CD8 antibody (AA 51-150)

Catalog No. ABIN671391

Product Details anti-CD8 Antibody

Target: CD8

Binding Specificity: AA 51-150

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD8 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse CD8

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CD8 antibody (ABIN671391)

Kovtun, Bergdolt, Hägele, Matthes, Lambris, Huber-Lang, Ignatius: "Complement receptors C5aR1 and C5aR2 act differentially during the early immune response after bone fracture but are similarly involved in bone repair." in: Scientific reports, Vol. 7, Issue 1, pp. 14061, (2019) (PubMed).

Wang, Sun, Zhang, Hu, Sun, Sheng, Fu: "Black tea and D. candidum extracts play estrogenic activity via estrogen receptor α-dependent signaling pathway." in: American journal of translational research, Vol. 10, Issue 1, pp. 114-125, (2018) (PubMed).

Rapp, Hachemi, Kemmler, Koenen, Tuckermann, Ignatius: "Induced global deletion of glucocorticoid receptor impairs fracture healing." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 32, Issue 4, pp. 2235-2245, (2018) (PubMed).

Schmid, Bouazzaoui, Schmid, Birner, Schach, Maier, Holler, Endemann: "Acute Renal Graft-Versus-Host Disease in a Murine Model of Allogeneic Bone Marrow Transplantation." in: Cell transplantation, Vol. 26, Issue 8, pp. 1428-1440, (2018) (PubMed).

Chuang, Jan, Chang, Lu: "The Chinese medicine JC-001 enhances the chemosensitivity of Lewis lung tumors to cisplatin by modulating the immune response." in: BMC complementary and alternative medicine, Vol. 17, Issue 1, pp. 210, (2017) (PubMed).

Wang, Johnson, He, Rong, Wong, Nigam, Behfar, Xu, Christman: "Humanized mouse model for assessing the human immune response to xenogeneic and allogeneic decellularized biomaterials." in: Biomaterials, Vol. 129, pp. 98-110, (2017) (PubMed).

Willett, Liu, Berke, Giannini, Schmid, Jia, Wang, Wang, Samson, Yu, Wang, Nawshad, Reinhardt: "Standardized Rat Model Testing Effects of Inflammation and Grafting on Extraction Healing." in: Journal of periodontology, Vol. 88, Issue 8, pp. 799-807, (2017) (PubMed).

Haffner-Luntzer, Fischer, Prystaz, Liedert, Ignatius: "The inflammatory phase of fracture healing is influenced by oestrogen status in mice." in: European journal of medical research, Vol. 22, Issue 1, pp. 23, (2017) (PubMed).

Kroner, Kovtun, Kemmler, Messmann, Strauss, Seitz, Schinke, Amling, Kotrba, Froebel, Dudeck, Dudeck, Ignatius: "Mast Cells Are Critical Regulators of Bone Fracture-Induced Inflammation and Osteoclast Formation and Activity." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 32, Issue 12, pp. 2431-2444, (2017) (PubMed).

Tanaka, Goshima, Esaki, Sato, Murata, Nishiyama, Watanabe, Kimura: "The efficacy of combination therapy with oncolytic herpes simplex virus HF10 and dacarbazine in a mouse melanoma model." in: American journal of cancer research, Vol. 7, Issue 8, pp. 1693-1703, (2017) (PubMed).

Prystaz, Kaiser, Kovtun, Haffner-Luntzer, Fischer, Rapp, Liedert, Strauss, Waetzig, Rose-John, Ignatius: "Distinct Effects of IL-6 Classic and Trans-Signaling in Bone Fracture Healing." in: The American journal of pathology, Vol. 188, Issue 2, pp. 474-490, (2017) (PubMed).

Jaime-Ramirez, Yu, Caserta, Yoo, Zhang, Lee, Hofmeister, Lee, Kumar, Pan, Kumar, Baiocchi, Teknos, Pichiorri, Kaur, Old: "Reolysin and Histone Deacetylase Inhibition in the Treatment of Head and Neck Squamous Cell Carcinoma." in: Molecular therapy oncolytics, Vol. 5, pp. 87-96, (2017) (PubMed).

Ashraf, Schwelberger, Brendel, Feurle, Andrassy, Kotsch, Regele, Pratschke, Maier, Aigner: "Exogenous Lipocalin 2 Ameliorates Acute Rejection in a Mouse Model of Renal Transplantation." in: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, Vol. 16, Issue 3, pp. 808-20, (2016) (PubMed).

Kovtun, Bergdolt, Wiegner, Radermacher, Huber-Lang, Ignatius: "The crucial role of neutrophil granulocytes in bone fracture healing." in: European cells & materials, Vol. 32, pp. 152-62, (2016) (PubMed).

Li, Kobayashi, Mona, Satomi, Okano, Inoue, Tani, Takahashi: "Immunogenic FEAT protein circulates in the bloodstream of cancer patients." in: Journal of translational medicine, Vol. 14, Issue 1, pp. 275, (2016) (PubMed).

Ju, Xing, Yang, Zheng, Jia, Zhang, Ren: "An effective cytokine adjuvant vaccine induces autologous T-cell response against colon cancer in an animal model." in: BMC immunology, Vol. 17, Issue 1, pp. 31, (2016) (PubMed).

Wang, Quan, Yan, Morales, Wetsel: "Targeted Disruption of the β2-Microglobulin Gene Minimizes the Immunogenicity of Human Embryonic Stem Cells." in: Stem cells translational medicine, Vol. 4, Issue 10, pp. 1234-45, (2015) (PubMed).

Target Details for CD8

Target: CD8

Alternative Name: CD8 (CD8 Products)

Synonyms: CD8 antibody, Leu2 antibody, MAL antibody, p32 antibody, CD8a molecule antibody, CD8A antibody

Background:

Synonyms: Ly-2, Ly-B, Ly-35, Lyt-2, BB154331, T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2, CD8a, Lyt2

Background: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Gene ID: 12525

UniProt: P01731