CD68 antibody (AA 21-120)

Catalog No. ABIN671406

Product Details anti-CD68 Antibody

Target: CD68 (CD68 Molecule (CD68))

Binding Specificity: AA 21-120

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD68 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunocytochemistry (ICC)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse CD68

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CD68 antibody (ABIN671406)

Guo, Jin, Jin, Chen, Lei, Wang: "Radical polymerization-crosslinking method for improving extracellular matrix stability in bioprosthetic heart valves with reduced potential for calcification and inflammatory response." in: Acta biomaterialia, Vol. 82, pp. 44-55, (2019) (PubMed).

Zhao, Yin, Fang, Xu, Sun, Xu, Liu, Peng: "Protective effects of dioscin against systemic inflammatory response syndromevia adjusting TLR2/MyD88/NF‑κb signal pathway." in: International immunopharmacology, Vol. 65, pp. 458-469, (2019) (PubMed).

Nishizawa, Iguchi, Fukuoka, Takahashi, Suda, Bando, Matsumoto, Yoshida, Odake, Ogawa, Takahashi: "IGF-I induces senescence of hepatic stellate cells and limits fibrosis in a p53-dependent manner." in: Scientific reports, Vol. 6, pp. 34605, (2018) (PubMed).

Ganguly, Sahu, Ohanyan, Haney, Chavez, Shah, Yalamanchili, Raman: "Oral chromium picolinate impedes hyperglycemia-induced atherosclerosis and inhibits proatherogenic protein TSP-1 expression in STZ-induced type 1 diabetic ApoE(-/-) mice." in: Scientific reports, Vol. 7, pp. 45279, (2017) (PubMed).

Kugo, Zaima, Onozato, Miyamoto, Hashimoto, Yanagimoto, Moriyama: "Suppressive effects of dietary EPA-rich fish oil on the degradation of elastin fibers in the aortic wall in nicotine-administered mice." in: Food & function, Vol. 8, Issue 8, pp. 2829-2835, (2017) (PubMed).

Vendrov, Stevenson, Alahari, Pan, Wickline, Madamanchi, Runge: "Attenuated Superoxide Dismutase 2 Activity Induces Atherosclerotic Plaque Instability During Aging in Hyperlipidemic Mice." in: Journal of the American Heart Association, Vol. 6, Issue 11, (2017) (PubMed).

Müller, Vogl, Pappritz, Miteva, Savvatis, Rohde, Most, Lassner, Pieske, Kühl, Van Linthout, Tschöpe: "Pathogenic Role of the Damage-Associated Molecular Patterns S100A8 and S100A9 in Coxsackievirus B3-Induced Myocarditis." in: Circulation. Heart failure, Vol. 10, Issue 11, (2017) (PubMed).

Xu, Yuan, Li, Dewey, Li, Zhang: "Lysosomal cholesterol accumulation in macrophages leading to coronary atherosclerosis in CD38(-/-) mice." in: Journal of cellular and molecular medicine, Vol. 20, Issue 6, pp. 1001-13, (2016) (PubMed).

Kang, Kim, Joung, Lee, You, Choi, Ryu, Ko, Lee, Lee, Ku, Shong, Lee, Kim: "The Roles of Adipokines, Proinflammatory Cytokines, and Adipose Tissue Macrophages in Obesity-Associated Insulin Resistance in Modest Obesity and Early Metabolic Dysfunction." in: PLoS ONE, Vol. 11, Issue 4, pp. e0154003, (2016) (PubMed).

Xu, Zhang, Halquist, Yuan, Henderson, Dewey, Li, Li, Zhang: "Simvastatin promotes NPC1-mediated free cholesterol efflux from lysosomes through CYP7A1/LXRα signalling pathway in oxLDL-loaded macrophages." in: Journal of cellular and molecular medicine, (2016) (PubMed).

Bang, Seo, Seo, Jo, Jung, Yu, Park, Park: "Bacillus subtilis KCTC 11782BP-Produced Alginate Oligosaccharide Effectively Suppresses Asthma via T-Helper Cell Type 2-Related Cytokines." in: PLoS ONE, Vol. 10, Issue 2, pp. e0117524, (2015) (PubMed).

Shang, Bai, Wang, Song, Guo, Zhang, Wang: "Delivery of Adipose-Derived Stem Cells Attenuates Adipose Tissue Inflammation and Insulin Resistance in Obese Mice Through Remodeling Macrophage Phenotypes." in: Stem cells and development, Vol. 24, Issue 17, pp. 2052-64, (2015) (PubMed).

Hong, Lee, Ko, Park, Park, Choi, Yoon, Ahn, Park, Woo, Oh: "Neonatal systemic inflammation in rats alters retinal vessel development and simulates pathologic features of retinopathy of prematurity." in: Journal of neuroinflammation, Vol. 11, pp. 87, (2014) (PubMed).

Wang, Hu, Wang, Gu, Gao, Cao, Zheng: "5-Aminolevulinic acid-mediated sonodynamic therapy reverses macrophage and dendritic cell passivity in murine melanoma xenografts." in: Ultrasound in medicine & biology, Vol. 40, Issue 9, pp. 2125-33, (2014) (PubMed).

Savvatis, Müller, Fröhlich, Pappritz, Zietsch, Hamdani, Grote, Schieffer, Klingel, Van Linthout, Linke, Schultheiss, Tschöpe: "Interleukin-6 receptor inhibition modulates the immune reaction and restores titin phosphorylation in experimental myocarditis." in: Basic research in cardiology, Vol. 109, Issue 6, pp. 449, (2014) (PubMed).

Xiao, Zou, Yang, Rehman, Ansari, Yuan, Zhou, Cui, Peng, Song: "The role of visfatin on the regulation of inflammation and apoptosis in the spleen of LPS-treated rats." in: Cell and tissue research, (2014) (PubMed).

Wang, Dong, Liu, Song, Song, Shang, Jiang, Guo, Zhang: "Programmed cell death-4 deficiency prevents diet-induced obesity, adipose tissue inflammation, and insulin resistance." in: Diabetes, Vol. 62, Issue 12, pp. 4132-43, (2014) (PubMed).

Zeng, Zhang, Li, Yang, Chen, Liu: "A new method to isolate and culture rat kupffer cells." in: PLoS ONE, Vol. 8, Issue 8, pp. e70832, (2013) (PubMed).

Chen, Bai, Liao, Peng, Wu, Wang, Zeng, Xie: "Electrospun poly(L-lactide)/poly(?-caprolactone) blend nanofibrous scaffold: characterization and biocompatibility with human adipose-derived stem cells." in: PLoS ONE, Vol. 8, Issue 8, pp. e71265, (2013) (PubMed).

Wu, Wu, Wu, Zhao, Li, Varghese, Moorhead, Powis, Chen, Ruan: "Chronic inflammation exacerbates glucose metabolism disorders in C57BL/6J mice fed with high-fat diet." in: The Journal of endocrinology, Vol. 219, Issue 3, pp. 195-204, (2013) (PubMed).

Target Details for CD68

Target: CD68 (CD68 Molecule (CD68))

Alternative Name: CD68 (CD68 Products)

Synonyms: Lamp4 antibody, Scard1 antibody, gp110 antibody, GP110 antibody, LAMP4 antibody, SCARD1 antibody, CD68 molecule antibody, CD68 antigen antibody, Cd68 molecule antibody, CD68 antibody, Cd68 antibody

Background:

Synonyms: Lamp4, gp11, Scard1, Macrosialin, CD68

Background: Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

Gene ID: 12514

UniProt: P31996