ATP-Binding Cassette, Sub-Family G (WHITE), Member 2 (ABCG2) antibody

Antigen: ATP-Binding Cassette, Sub-Family G (WHITE), Member 2 (ABCG2)

Synonyms: MRX, MXR, ABCP, BCRP, BMDP, MXR1, ABC15, BCRP1, CD338, CDw338, EST157481, MGC102821, Bcrp1, AI428558, ABCG2, MGC127688, bcrp

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus), Mouse (Murine)

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Flow Cytometry (FACS), Immunofluorescence (IF)

Catalog no.: ABIN671601

Additional Information

Alternative name: ABCG2

Immunogen: Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from human ABCG2

Format: Lyophilized

Reconstitution: Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)

Isotype: IgG  (Matching secondary antibodies)

Description: Synonym: BCRP, BXP-21, ABC15, ABCG 2, ABCG2, ABCP, ATP-binding cassette sub-family G member 2, BCRP1, BMDP, Breast cancer resistance protein, CDw338, CDw338, CD338, antigen, EST157481, MGC102821, Mitoxantrone resistance associated protein, MRX, MXR, MXR1, Placenta-specific ATP-binding cassette transporter.

Molecular Weight: 72kDa

Comments:

An ABC transporter. Allows efflux of Hoechst dye

a property that has been used to separate bone marrow side population cells

which express BCRP/ABCG2. Appears to play a major role in the multidrug resistance phenotype of a specific MCF-7 breast cancer cell line. When overexpressed

the transfected cells become resistant to mitoxantrone

daunorubicin and doxorubicin

display diminished intracellular accumulation of daunorubicin

and manifest an ATP-dependent increase in the efflux of rhodamine 123.Breast Cancer Resistance Protein (BCRP) is a 70 kDa ATP-Binding Cassette membrane transport protein involved in multidrug resistance. BCRP may be over-expressed in cancer cell lines selected with doxorubicin / verapamil

topotecan or mitoxantrone.

Application Details

Application Notes: WB (1:100-500), ELISA (1:500-1000), IP (1:20-100), IHC-P (1:100-500), IHC-F (1:100-500), Flow-Cyt (1:20-100), IF (1:50-200)

Concentration: 1mg/1ml

Purification: affinity purified by Protein A

Buffer: 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

Storage: Ship at room temperature. Store at -20°C

Research Area: Transporters

Restrictions: For Research Use only