Aggrecan antibody (AA 101-220)

Catalog No. ABIN673599

Product Details anti-Aggrecan Antibody

Target: Aggrecan (ACAN)

Binding Specificity: AA 101-220

Reactivity: Human, Rat, Mouse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aggrecan antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rabbit, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Aggrecan core protein

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Aggrecan antibody (ABIN673599)

Hu, Yang, Chen, Sun, Lin, Yang: "Optimization of puncture injury to rat caudal disc for mimicking early degeneration of intervertebral disc." in: Journal of orthopaedic research : official publication of the Orthopaedic Research Society, (2017) (PubMed).

Zhao, Hao, Wang, Wang: "Effect of Choline on the Composition and Degradation Enzyme of Extracellular Matrix of Mice Chondrocytes Exposed to Fluoride." in: Biological trace element research, (2016) (PubMed).

Liu, Yu, Ma, Xiang, Hu, Chen: "Lentivirus-mediated TGF-β3, CTGF and TIMP1 gene transduction as a gene therapy for intervertebral disc degeneration in an in vivo rabbit model." in: Experimental and therapeutic medicine, Vol. 11, Issue 4, pp. 1399-1404, (2016) (PubMed).

Shi, Ma, Zhang, Li, Jiang, Qin: "Hypoxia combined with spheroid culture improves cartilage specific function in chondrocytes." in: Integrative biology : quantitative biosciences from nano to macro, (2015) (PubMed).

Lei, Li, Li, Gao, Chen, Jin: "Mesenchymal stem cell characteristics of dental pulp and periodontal ligament stem cells after in vivo transplantation." in: Biomaterials, Vol. 35, Issue 24, pp. 6332-43, (2014) (PubMed).

Ren, Liu, Ma, Liu, Diao, Yang, Zhang, Xi, Hu: "Treatment of rabbit intervertebral disc degeneration with co-transfection by adeno-associated virus-mediated SOX9 and osteogenic protein-1 double genes in vivo." in: International journal of molecular medicine, Vol. 32, Issue 5, pp. 1063-8, (2014) (PubMed).

Target Details for Aggrecan

Target: Aggrecan (ACAN)

Alternative Name: Aggrecan core protein 1 (ACAN Products)

Synonyms: AGC1 antibody, AGCAN antibody, CSPG1 antibody, CSPGCP antibody, MSK16 antibody, SEDK antibody, Agc antibody, Agc1 antibody, Cspg1 antibody, b2b183Clo antibody, cmd antibody, aggrecan antibody, aggrecan core protein antibody, ACAN antibody, Acan antibody, LOC100380783 antibody, AGC antibody

Background:

Synonyms: AGC1, SEDK, AGCAN, CSPG1, MSK16, CSPGCP, Aggrecan core protein, Cartilage-specific proteoglycan core protein, CSPCP, Chondroitin sulfate proteoglycan core protein 1, Chondroitin sulfate proteoglycan 1, ACAN

Background: This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.

Gene ID: 176

UniProt: P16112

Pathways: Glycosaminoglycan Metabolic Process, Dicarboxylic Acid Transport