Fibrinogen antibody

Details for Product anti-Fibrinogen Antibody No. ABIN673854, Supplier: Log in to see
Antigen
  • fib2
  • MGC53620
  • fibrinogen
  • sb:cb892
  • wu:fa55c12
  • wu:fb20e08
  • wu:fb59b03
  • wu:fi39d04
  • zgc:92117
  • fibrinogen alpha chain
  • fibrinogen gamma chain
  • fga
  • FGA
  • CpipJ_CPIJ010090
  • FGG
Reactivity
Human
315
87
57
33
28
24
14
4
1
1
1
Host
Rabbit
158
157
129
63
9
3
Clonality
Polyclonal
Conjugate
This Fibrinogen antibody is un-conjugated
46
46
46
7
6
6
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
Application
ELISA
331
257
55
54
52
42
40
38
35
33
28
27
20
19
15
9
8
6
4
3
2
2
2
1
1
1
1
1
1
1
1
1
Options
Supplier
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Available images

'Independent Validation' Badge
Lot Number 980680
Method validated ELISA
Positive Control Liver
Negative Control Skeletal muscle
Notes Signal was detected in positive control samples but not in negative control samples.
Primary Antibody
  • Antibody: Fibrinogen antibody
  • Catalog number: ABIN673854
  • Batch number: 980680
Controls
  • Positive control: protein extract from human liver (specimen known to contain the target protein) was from Alamo Laboratories, Inc (Cat # 2001-TEL-Hu).
  • Negative control: protein extract from mouse skeletal muscle (specimen known to not contain the target protein) was from Alamo Laboratories, Inc (Cat # 2002-TEM-Mm).
  • Standard curve: serial two-fold dilutions from 200 ng/ml [200, 100, 50, 25, 12.5, 6.25, 3.125, 1.5625, 0.78125, 0] were generated from Fibrinogen peptide stock diluted in 1 x 50 mM Carbonate buffer, pH 9.5.
  • Spike control: standard diluted in protein lysate buffer [25 and 0].
Protocol
  • A 96-well MICROLON® 600 96W High Binding 12 x 8 Clear Strip Microplate was coated with antigen by pipetting 100 µL of sample per well. All samples and standards were assayed in triplicate.
  • The microplate was covered and incubated at 25°C overnight.
  • After overnight coating, plate contents were discarded and wells were washed 3 times with 150 µL of 1 x PBST (PBS, 0.1% Tween-20) per well.
  • Wells were then blocked with 250 µL of 1 x PBS / 0.05% Tween-20 / 1% BSA per well and incubated at 37°C for 2 h.
  • After blocking, plate contents were discarded and wells were washed 3 times with 250 µL of 1 x PBS per well.
  • 100 µL of primary antibody diluted 1:500 in 1 x PBS / 0.05% Tween-20 / 1% BSA was added per well and incubated at 37°C for 2 h.
  • Plate contents were discarded and wells were washed 3 times with 200 µL of 1 x PBST per well.
  • 100 µL of HRP conjugated secondary antibody diluted 1:10000 in 1 x PBS / 0.05% Tween-20 / 1% BSA was added per well and incubated at 37°C for 1 h.
  • Plate contents were discarded and wells were washed 3 times with 200 µL of 1 x PBST per well.
  • 100 µL of TMB (3,3', 5,5"-tetramethylbenzidine) substrate was added per well and incubated at 37°C for 10 min.
  • 100 µL of STOP solution was added per well.
  • The optical density (OD value) of each well was read using a microplate reader set to 450 nm.
  • The triplicate readings for each sample were averaged and the average zero standard optical density subtracted. The corrected average-value was tabulated as Average Absorbance. A standard curve was generated by plotting the mean OD value for each standard on the y-axis against the concentration on the x-axis using Excel. A line of best fit through the points on the graph was used to generate the equation X = (Y-0.0122) / 0.0113.
  • The equation X = (Y-0.0122) / 0.0113 was used to calculate fibrinogen concentrations of the samples based on their Average Absorbance values.
Experimental Notes None
Validation Images
ELISA validation image for anti-Fibrinogen antibody (ABIN673854) Figure 1: Graph of corrected-average absorbance (OD 450 nm) readings plotted for stan...
ELISA validation image for anti-Fibrinogen antibody (ABIN673854) Table 1: ELISA. Fibrinogen is present in the positive control sample (liver) and abse...
Immunogen Fibrinogen from human plasma
Isotype IgG
Purification Purified by Protein A.
Alternative Name Human Fibrinogen
Background

Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the immune response via both innate and T-cell mediated pathways.

Synonyms: Fib2, Fibrinogen alpha chain, FGA

Gene ID 2243
UniProt P02671
Application Notes ELISA 1:500-1000
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months
Supplier Images
ELISA image for anti-Fibrinogen antibody (ABIN673854) Antigen: human liver Primary:Rabbit Anti-human Fibrinogen Polyclonal Antibody, 1:500 ...
Product cited in: Chen, Yang, Liao, Wang, Chen, Sun, Huang: "Effect of the duration of UV irradiation on the anticoagulant properties of titanium dioxide films." in: ACS applied materials & interfaces, Vol. 7, Issue 7, pp. 4423-32, 2015 (PubMed).

Chen, Zhao, Chen, Liao, Yang, Sun, Huang: "The effect of full/partial UV-irradiation of TiO2 films on altering the behavior of fibrinogen and platelets." in: Colloids and surfaces. B, Biointerfaces, Vol. 122, pp. 709-18, 2014 (PubMed).