NRF1 antibody (AA 51-180)

Catalog No. ABIN675219

Product Details anti-NRF1 Antibody

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Binding Specificity: AA 51-180

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NRF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NRF1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-NRF1 antibody (ABIN675219)

Yan, Ji, Shi, Li, Sang: "Acute nitrogen dioxide inhalation induces mitochondrial dysfunction in rat brain." in: Environmental research, Vol. 138, pp. 416-24, (2015) (PubMed).

Qin, Wang, Huo, Yan, Jiang, Zhou, Wang, Sang: "Sulfur dioxide inhalation stimulated mitochondrial biogenesis in rat brains." in: Toxicology, Vol. 300, Issue 1-2, pp. 67-74, (2012) (PubMed).

Target Details for NRF1

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Alternative Name: NRF-1 (NRF1 Products)

Background:

Synonyms: ALPHA-PAL, Nuclear respiratory factor 1, NRF-1, Alpha palindromic-binding protein, NRF1

Background: Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.

Gene ID: 4899

UniProt: Q16656

Pathways: Regulation of Lipid Metabolism by PPARalpha