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Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) antibody (PE)

Antigen

Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B)
Synonyms Darpp32, AU040756, DARPP-32, DARPP32, FLJ20940, Darpp-32, darpp32, darpp-32, PPP1R1B, MGC139723
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Rat (Rattus), Mouse (Murine)
Conjugate
Application
Alternatives Flow Cytometry (FACS), Immunofluorescence (IF)
Catalog no. ABIN680333
Quantity 100 µg  (1mg/1ml)
Price 330.00 $   Plus shipping costs $35.00
Shipping to
Availability Ships within 10 to 15 Business Days

Additional Information
Application Details Alternatives back to top

Alternative name DARPP32
Immunogen Polyclonal antibodies are produced by immunizing animals with synthetic peptide from the human DARPP32 conjugated to KLH
Format Lyophilized
Reconstitution Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)
Isotype IgG
Description Synonym: DARPP 32, DARPP-32, Dopamine and cAMP regulated neuronal phosphoprotein, Dopamine and cAMP regulated phosphoprotein DARPP 32, Dopamine and cAMP regulated phosphoprotein DARPP32, FLJ20940, IPPD, Neuronal phosphoprotein DARPP 32, PPP1R1B, Protein phosphatase 1 regulatory (inhibitor) subunit 1B, Protein phosphatase 1 regulatory subunit 1B.
Specificity Excitation / Emission Wavelengths: 565nm/578nm
Molecular Weight 32kDa
Comments

DARPP32 is expressed in medium sized spiny neurons that also express dopamine D1 receptors. The function of DARPP32 seems to be regulated by receptor stimulation. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation

but in opposite directions. Dopamine D1 receptor stimulation enhances cAMP formation

resulting in the phosphorylation of DARPP32 and phosphorylated DARPP32 is a potent protein phosphatase 1. NMDA receptor stimulation elevates intracellular calcium

which leads to activation of calcineurin and dephosphorylation of phospho DARPP32

thereby reducing the phosphatase 1 inhibitory activity of DARPP32.

Application Details
Alternatives back to top

Application Notes Flow-Cyt (1:50-200), IF (1:50-200)
Concentration 1mg/1ml
Purification Antibodies are purified by protein A and peptide affinity chromatography.
Buffer 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
Storage Ship at room temperature. Store at -20°C
Research Area Signaling
Restrictions For Research Use only

Alternatives
Application Details back to top

Alternatives for antigen "Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B)", type "Antibodies"
Hosts Rabbit (48), Mouse (4)
Reactivities Human (43), Rat (Rattus) (37), Mouse (Murine) (34), Cow (Bovine) (12), Dog (Canine) (7), Chicken (6), Rabbit (3), Monkey (2), Xenopus laevis (2), Pig (Porcine) (1)
Applications Western Blotting (WB) (33), Immunofluorescence (IF) (22), Flow Cytometry (FACS) (19), ELISA (7), Immunohistochemistry (IHC) (7), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Enzyme Immunoassay (EIA) (3), Immunoprecipitation (IP) (3), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
Conjugates Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
Epitopes pThr75 (8), pThr34 (6), Ab-34 (1), Ab-75 (1), N-Term (1)