C3 antibody (AA 1551-1663)

Catalog No. ABIN681913

Product Details anti-C3 Antibody

Target: C3 (Complement Component 3 (C3))

Binding Specificity: AA 1551-1663

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Cow,Pig,Horse,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Complement C3 alpha chain

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-C3 antibody (ABIN681913)

Rogińska, Kawa, Pius-Sadowska, Lejkowska, Łuczkowska, Wiszniewska, Kaarniranta, Paterno, Schmidt, Machaliński, Machalińska: "Depletion of the Third Complement Component Ameliorates Age-Dependent Oxidative Stress and Positively Modulates Autophagic Activity in Aged Retinas in a Mouse Model." in: Oxidative medicine and cellular longevity, Vol. 2017, pp. 5306790, (2018) (PubMed).

Zhuang, Han, Li, Kienhöfer, Lee, Shumyak, Meyerholz, Rosadzinski, Rosner, Chan, Xu, Segal, Sobel, Yang, Hoffmann, Reeves: "A Novel Mechanism for Generating the Interferon Signature in Lupus: Opsonization of Dead Cells by Complement and IgM." in: Arthritis & rheumatology (Hoboken, N.J.), Vol. 68, Issue 12, pp. 2917-2928, (2016) (PubMed).

Trang, Hirai, Nabeta, Fuke, Yamaguchi: "Membranoproliferative glomerulonephritis in a calf with nephrotic syndrome." in: Journal of comparative pathology, Vol. 151, Issue 2-3, pp. 162-5, (2014) (PubMed).

Target Details for C3

Target: C3 (Complement Component 3 (C3))

Alternative Name: Complement C3 (C3 Products)

Synonyms: AHUS5 antibody, ARMD9 antibody, ASP antibody, C3a antibody, C3b antibody, CPAMD1 antibody, AI255234 antibody, HSE-MSF antibody, Plp antibody, C3 antibody, C3-1 antibody, sb:cb26 antibody, si:dkey-76b14.4 antibody, wu:fi34b03 antibody, wu:fj86e11 antibody, C3d antibody, asp antibody, cpamd1 antibody, c3 antibody, complement C3 antibody, complement component 3 antibody, complement component c3a, duplicate 1 antibody, complement component 3 L homeolog antibody, complement component C3 antibody, complement C3-like antibody, C3 antibody, c3a.1 antibody, c3.L antibody, LOC443475 antibody, c3 antibody, c3-1 antibody, LOC100060539 antibody

Background:

Synonyms: ASP, C3a, C3b, AHUS5, ARMD9, CPAMD1, HEL-S-62p, Complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, C3

Background: C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C3-beta-c: Acts as a chemoattractant for neutrophils in chronic inflammation. Acylation stimulating protein: adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2 (PubMed:837664, PubMed:29953, PubMed:959512, PubMed:1432298, PubMed:15833747, PubMed:16333141, PubMed:1961575).

Gene ID: 718

UniProt: P01024

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling