AIF1 antibody (Allograft Inflammatory Factor 1) (AA 116-147)

Details for Product anti-AIF1 Antibody No. ABIN685477, Supplier: Log in to see
Antigen
  • fa04b11
  • wu:fa04b11
  • AIF1
  • aif1
  • AIF-1
  • IBA1
  • IRT-1
  • IRT1
  • Bart1
  • iba1
  • mrf-1
  • AI607846
  • D17H6S50E
  • G1
  • Iba1
  • AIF
  • AIF1L
  • allograft inflammatory factor 1
  • Allograft inflammatory factor 1
  • allograft inflammatory factor 1-like
  • aif1
  • AIF1
  • Aif1
  • LOC100350092
  • C9ORF58
Alternatives
anti-Rat (Rattus) AIF1 antibody for ELISA
Epitope
AA 116-147
37
25
20
14
14
14
10
8
8
6
4
4
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
206
105
96
8
7
2
2
1
1
Host
Rabbit
138
56
34
2
1
Clonality
Polyclonal
Conjugate
This AIF1 antibody is un-conjugated
23
22
11
5
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
181
123
104
65
28
27
19
12
10
10
7
4
2
2
2
2
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see

Available images

'Independent Validation' Badge
Lot Number 980993W
Method validated Immunofluorescence
Positive Control Mouse spleen
Negative Control Mouse duodenum
Notes Strong signal was observed in the positive control tissue, while no signal was observed in the negative control tissue.
Primary Antibody
Isotype Control Antibody
Secondary Antibody
Controls
  • Positive control: Wild type mouse spleen (specimen known to contain the target protein) from Explora BioLabs.
  • Negative Control: Wild type mouse duodenum (specimen known to not contain the target protein) from Explora BioLabs.
  • Primary antibody isotype control: Wild type mouse spleen treated with primary antibody isotype control instead of the primary antibody.
  • Secondary antibody only control: Wild type mouse spleen treated with secondary antibody only (no primary antibody).
Protocol
  • Frozen, OCT embedded tissues were cut 5 µm and mounted on positive charged slides.
  • Sections were air dried for 30 min prior to fix in 10% neutral buffered formalin for 10 min.
  • Fixed slides were rinsed three times in PBS for 5 min each at RT.
  • Sections were blocked in 1 X PBS / 3% donkey serum / 0.1% Triton X-100 for 10 min at RT.
  • Sections were incubated with primary antibody diluted 1:100 in Universal Antibody Dilution Buffer (Electron Microscopy Sciences, 25886-05) overnight at 4°C.
  • Sections were rinsed three times in PBS for 5 min each at RT.
  • Sections were incubated with secondary antibody diluted 1:200 in Universal Antibody Dilution Buffer (Electron Microscopy Sciences, 25886-05) for 120 min at RT in dark.
  • Sections were mounted on slides with FluoroGel II with DAPI (Electron Microscopy Sciences).
  • Stained sections were imaged with a 3D Histech Pannoramic SCAN 150.
Experimental Notes None
Validation Images
Immunofluorescence validation image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 116-147) antibody (ABIN685477) Figure 1: AIF1 staining on mouse spleen (red), counterstained with DAPI (blue).
Immunofluorescence validation image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 116-147) antibody (ABIN685477) Figure 2: AIF1 staining on mouse duodenum (red), counterstained with DAPI (blue).
Immunofluorescence validation image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 116-147) antibody (ABIN685477) Figure 3: Isotype control staining on mouse spleen (red), counterstained with DAPI (b...
Immunofluorescence validation image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 116-147) antibody (ABIN685477) Figure 4: Secondary only staining on mouse spleen (red), counterstained with DAPI (bl...
Immunogen KLH conjugated synthetic peptide derived from human AIF1
Isotype IgG
Purification Purified by Protein A.
Alternative Name Aif1 (AIF1 Antibody Abstract)
Background

Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.

Subcellular location: Cytoplasm, Membrane bound

Synonyms: IBA1, IRT1, AIF-1, IRT-1, Allograft inflammatory factor 1, Ionized calcium-binding adapter molecule 1, Protein G1, AIF1, G1

Gene ID 199
UniProt P55008
Research Area Inflammation
Pathways Smooth Muscle Cell Migration
Application Notes WB 1:100-1000
IHC-P 1:100-500
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-AIF1 antibody (Allograft Inflammatory Factor 1) (AA 116-147) (ABIN685477) Formalin-fixed and paraffin embedded mouse brain labeled with Anti-AIF1/IBa-1 Polyclo...
Immunohistochemistry (IHC) image for anti-AIF1 antibody (Allograft Inflammatory Factor 1) (AA 116-147) (ABIN685477) Formalin-fixed and paraffin embedded rat brain labeled with Anti-AIF1/IBa-1 Polyclona...
Immunofluorescence (IF) image for anti-AIF1 antibody (Allograft Inflammatory Factor 1) (AA 116-147) (ABIN685477) Independently Validated Antibody, image provided by Science Direct, badge number 0296...
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) image for anti-AIF1 antibody (Allograft Inflammatory Factor 1) (AA 116-147) (ABIN685477) Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-AIF1 Polyclon...
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) image for anti-AIF1 antibody (Allograft Inflammatory Factor 1) (AA 116-147) (ABIN685477) Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-AIF1 Polyclon...
Product cited in: Hsu, Yao, Hsu, Lin, Chen, Chen: "Effects of Taxol on Regeneration in a Rat Sciatic Nerve Transection Model." in: Scientific reports, Vol. 7, pp. 42280, 2017 (PubMed). Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Rat (Rattus)).

Fu, Xia, Liu, Wang, Wang, Shi, Song, Song, Song et al.: "The acute exposure of tetrachloro-p-benzoquinone (a.k.a. chloranil) triggers inflammation and neurological dysfunction via Toll-like receptor 4 signaling: The protective role of melatonin ..." in: Toxicology, Vol. 381, pp. 39-50, 2017 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)).

Ogawa, Onozato, Okuhara, Nagasawa, Tamura, Hayashi: "Spontaneous cutaneous soft tissue sarcoma with differentiation into fibroblasts in a Sprague-Dawley rat." in: Journal of toxicologic pathology, Vol. 29, Issue 2, pp. 119-24, 2016 (PubMed).