Lipocalin 2 antibody (AA 101-198)

Catalog No. ABIN685627

Product Details anti-Lipocalin 2 Antibody

Target: Lipocalin 2 (LCN2)

Binding Specificity: AA 101-198

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Lipocalin 2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Lipocalin 2

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Lipocalin 2 antibody (ABIN685627)

Xu, Zhang, Cheng, Liu, Zou, Zhan, Xiao, Xia: "Transcription factor 7-like 1 dysregulates keratinocyte differentiation through upregulating lipocalin 2." in: Cell death discovery, Vol. 2, pp. 16028, (2016) (PubMed).

Xing, Wang, Cheng, Montaner, Mandeville, Leung, van Leyen, Lok, Wang, Lo: "Neuronal production of lipocalin-2 as a help-me signal for glial activation." in: Stroke; a journal of cerebral circulation, Vol. 45, Issue 7, pp. 2085-92, (2014) (PubMed).

Target Details for Lipocalin 2

Target: Lipocalin 2 (LCN2)

Alternative Name: Lipocalin 2 (LCN2 Products)

Synonyms: LCN2 antibody, 24p3 antibody, MSFI antibody, NGAL antibody, AW212229 antibody, Sip24 antibody, lipocalin 2 antibody, LCN2 antibody, Lcn2 antibody

Background:

Synonyms: 24p3, MSFI, NGAL, Neutrophil gelatinase-associated lipocalin, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, Lipocalin-2, Oncogene 24p3, Siderocalin LCN2, p25, LCN2, HNL

Background: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.

Gene ID: 3934

UniProt: P80188

Pathways: Cellular Response to Molecule of Bacterial Origin, Transition Metal Ion Homeostasis