Laminin, beta 1 (LAMB1) antibody

Antigen: Laminin, beta 1 (LAMB1)

Synonyms: CLM, MGC142015, gup, fc20b05, sb:cb95, wu:fc20b05, Lamb1-1, LAMB1, lams, lamb2

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus), Mouse (Murine), Rabbit, Horse (Equine), Chicken, Dog (Canine)

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Flow Cytometry (FACS), Immunofluorescence (IF)

Catalog no.: ABIN687787

Additional Information

Alternative name: Laminin Beta 1

Immunogen: Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from human Laminin beta 1 C-terminus

Format: Lyophilized

Reconstitution: Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)

Isotype: IgG  (Matching secondary antibodies)

Description: Synonym: LAMA 1 LAMA, LAMA1, LAMB1, Laminin A chain, Laminin alpha 1, Laminin alpha 1 chain, Laminin B1 chain, Laminin subunit beta 1.

Molecular Weight: 196kDa

Comments:

Laminins

a family of extracellular matrix glycoproteins

are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion

differentiation

migration

signaling

neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha

beta and gamma (formerly A

B1

and B2

respectively) and they form a cruciform structure consisting of 3 short arms

each formed by a different chain

and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha

beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery

i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown

but some of the chains have been shown to differ with respect to their tissue distribution

presumably reflecting diverse functions in vivo. This gene encodes the beta chain isoform laminin

beta 1. The beta 1 chain has 7 structurally distinct domains which it shares with other beta chain isomers. The C-terminal helical region containing domains I and II are separated by domain alpha

domains III and V contain several EGF-like repeats

and domains IV and VI have a globular conformation. Laminin

beta 1 is expressed in most tissues that produce basement membranes

and is one of the 3 chains constituting laminin 1

the first laminin isolated from Engelbreth-Holm-Swarm(EHS) tumor. A sequence in the beta 1 chain that is involved in cell attachment

chemotaxis

and binding to the laminin receptor was identified and shown to have the capacity to inhibit metastasis.

Application Details

Application Notes: WB (1:100-500), ELISA (1:500-1000), IP (1:20-100), IHC-P (1:100-500), IHC-F (1:100-500), Flow-Cyt (1:20-100), IF (1:50-200)

Concentration: 1mg/1ml

Purification: affinity purified by Protein A

Buffer: 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

Storage: Ship at room temperature. Store at -20°C

Restrictions: For Research Use only