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Amphiregulin (AREG) antibody (Biotin)

Antigen

Amphiregulin (AREG)

Synonyms AR, SDGF, CRDGF, MGC13647, Sdgf, AREG, AREGB, MGC152310
Clonality Polyclonal
Host
Alternatives

Rabbit

Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus), Rabbit, Horse (Equine), Cow (Bovine), Sheep (Ovine), Dog (Canine)

Conjugate
Alternatives Biotin
Application
Alternatives Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Flow Cytometry (FACS), Immunofluorescence (IF)
Catalog no. ABIN704998
Quantity 100 µg  (1mg/1ml)
Price 290.00 $   Plus shipping costs $35.00
Shipping to
Availability Ships within 10 to 15 Business Days

Additional Information

Alternative name Amphiregulin
Immunogen Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from human Amphiregulin C-terminus
Format Lyophilized
Reconstitution Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)
Isotype IgG
Description Synonym: AR, Colorectum cell derived growth factor, CRDGF, A REG, AR antibody, AREG, Colorectum cell derived growth factor, CRDGF, MGC13647, Schwannoma derived growth factor, SDGF.
Molecular Weight 28kDa
Comments

Amphiregulin expression is induced by phorbol ester

estrogen

androgen

and other EGFR ligands. In vitro

amphiregulin functions as an autocrine growth stimulator to drive proliferation of colon carcinoma cells

normal and oncogene-transformed mammary epithelial cells

cervical carcinoma cells

prostate cancer cells

and keratinocytes. Amphiregulin is important in the development of human placenta and murine mammary gland. It is reportedly overexpressed in human cancers of breast

colon

stomach and pancreas.

Application Details

Application Notes WB (1:100-500), ELISA (1:500-1000), IP (1:20-100), IHC-P (1:100-500), IHC-F (1:100-500), Flow-Cyt (1:20-100), IF (1:50-200)
Concentration 1mg/1ml
Purification Antibodies are purified by protein A and peptide affinity chromatography.
Buffer 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
Storage Ship at room temperature. Store at -20°C
Research Area Cancer, Growth Factors
Restrictions For Research Use only

Alternatives

Alternatives for antigen "Amphiregulin (AREG)", type "Antibodies"
Hosts Rabbit (21), Mouse (2)
Reactivities Human (23), Mouse (Murine) (21), Rat (Rattus) (19), Cow (Bovine) (16), Horse (Equine) (16), Dog (Canine) (15), Rabbit (15), Sheep (Ovine) (15), Pig (Porcine) (3)
Applications Flow Cytometry (FACS) (17), Immunofluorescence (IF) (17), Western Blotting (WB) (4), ELISA (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Immunoprecipitation (IP) (2), ELISA (Detection) (1), Enzyme Immunoassay (EIA) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (1)
Conjugates Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
Epitopes AA 8-26 (1)