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TNFSF9 antibody (AA 101-200)

TNFSF9 Reactivity: Human, Mouse, Rat WB, ELISA, ICC, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN705056
  • Target See all TNFSF9 Antibodies
    TNFSF9 (Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))
    Binding Specificity
    • 14
    • 8
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 101-200
    Reactivity
    Human, Mouse, Rat
    Host
    • 41
    • 18
    • 7
    • 2
    Rabbit
    Clonality
    • 36
    • 29
    • 2
    Polyclonal
    Conjugate
    • 39
    • 5
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This TNFSF9 antibody is un-conjugated
    Application
    • 47
    • 33
    • 28
    • 12
    • 12
    • 8
    • 6
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Cross-Reactivity
    Human, Mouse, Rat
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human TNFSF9
    Isotype
    IgG
  • Application Notes
    WB 1:300-5000
    ELISA 1:500-1000
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    ICC 1:100-500
    Restrictions
    For Research Use only
  • Validation #029578 (Immunohistochemistry)
    'Independent Validation' Badge
    by
    Immunohistochemistry Core, NYU Langone
    No.
    #029578
    Date
    01/18/2014
    Antigen
    Lot Number
    999892W
    Method validated
    Immunohistochemistry
    Positive Control
    Human liver
    Notes
    Signal was detected in positive control tissue and not in negative control tissue.
    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    • Antibody: Tumor necrosis factor ligand superfamily member 9 (TNFSF9)
    • Catalog number: ABIN705056
    • Lot number: 999892W
    Secondary Antibody
    • Antibody: Biotinylated goat anti-rabbit/anti-mouse (Kit)
    • Lot number: D05923BA
    Full Protocol
    • Immunohistochemistry was performed on a Ventana NexES automated platform, instrument manufacturer specific reagents are italicized.
    • 1. Slides were preheated in convection oven at 60°C for 30 minutes
    • 2. Deparaffinization procedure: - 3 changes of Xylene, 5 minutes each - 3 changes of 100% Ethanol, 3 minutes each - 3 changes of 95% Ethanol, 3 minutes each - Rinsed in distilled water, 3 changes
    • 1. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 minutes. - Slides were allowed to cool (in citrate) for 30 minutes. - Slides were washed x 3 in Distilled water
    • 1. NexES instrument procedure, iVIEW DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
    • 1. Slides rinsed with *reaction buffer* x 3
    • 1. *iVIEW Inhibitor (H2O2)* applied and incubated for 4 minutes
    • 1. Slides rinsed with *reaction buffer*
    • 1. Antibody Application - Primary antibody diluted 1:250 in PBS (100 microliters applied/slide) - Ventana Isotype control applied neat - Slides incubated overnight at room temperature (~12 hours ~25°C)
    • 1. Slides rinsed with *reaction buffer* x3
    • 1. *iVIEW Biotinylated IgG* applied and incubated for 8 minutes
    • 1. Slides rinsed with *reaction buffer*
    • 1. *iVIEW Streptavidin-Horseradish Peroxidase* applied and incubated for 8 minutes
    • 1. Slides rinsed with *reaction buffer*
    • 1. *iVIEW DAB/H2O2* applied and incubated for 8 minutes
    • 1. Slides rinsed with *reaction buffer*
    • 1. *iVIEW Copper* applied and incubated for 4 minutes
    • 1. Slides rinsed with *reaction buffer*
    • 1. Slides washed in Dawn Detergent/tap water
    • 1. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 seconds - Slides washed in tap water, 1 minute - Decolorized (10% Acetic Acid in 70% ethanol), 1 minute - Slides washed in tap water, 1 minute - Bluing (Austin Clear Ammonia), 1 minute - Slides washed in tap water, 1 minute
    • 1. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 minutes each - 3 changes of 100% Ethanol, 3 minutes each - 3 changes of Xylene, 5 minutes each - Mounted with Permount
    • 1. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
    Experimental Notes
    • Deviations from protocol/procedure supplied by manufacturer:
    • Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
    • Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
    • Step 3.1: Slides heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
    • Step 3.2: Slides cooled for 30 minutes; manufacturer cools for 20 minutes.
    • Step 4: Italicized reagents and incubation time are fixed instrument parameters.
    • Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
    • Step 8.1: Antibody diluted in PBS at 1:250; manufacture recommends dilution range (1:100-1:500). No primary antibody diluent recommended.
    • Step 8.2.1: Primary antibody incubated at room temperature overnight; manufacturer incubates overnight 4°C with agitation.
    • Tissue Interpretation (limited): • TNFSF9: Under the staining parameters described above, placenta was weakly cytoplasmic positive (trophoblastic and decidua cells). Liver hepatocytes were weakly stained. Substantial signal detected in limited number of other tissues, including kidney, prostate, and skin.
    • • I-NC (Isotype negative control): No signal detected
    • • B-NC (Blank negative control): No signal detected
    • Signal Localization: - Signal to noise was adequate with predominately cytoplasmic subcellular localization observed. Nuclear staining was rare and no distinct membrane signal was detected.
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Expiry Date
    12 months
  • Target
    TNFSF9 (Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))
    Alternative Name
    TNFSF9/CD137L (TNFSF9 Products)
    Synonyms
    TNFSF9 antibody, 4-1BB-L antibody, 4-1BBL antibody, AI848817 antibody, Cd137l antibody, Ly63l antibody, CD137L antibody, TNF superfamily member 9 antibody, tumor necrosis factor (ligand) superfamily, member 9 antibody, Tnfsf9 antibody, LOC476729 antibody, TNFSF9 antibody
    Background

    Synonyms: CD137L, 4-1BB-L, Tumor necrosis factor ligand superfamily member 9, 4-1BB ligand, 4-1BBL, TNFSF9

    Background: Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages.

    Gene ID
    8744
    UniProt
    P41273
    Pathways
    Activated T Cell Proliferation, Cancer Immune Checkpoints
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